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Electron microscopic analysis of autophagy in tumors and metabolic disorders
Serbia (IFMSA-Serbia) - University of Belgrade, Belgrade
Institute of Histology and Embryology, Medical School, University of Belgrade
Assistant Professor Tamara Kravic
Teaching Assistant Tamara Martinovic
Type of Research Project
- Basic science
What is the background of the project?
Autophagy promotes tumor cell survival and drug resistance in various types of tumors. We had previously analyzed the ultrastructural features of peripheral neuroblastic tumors, identified, and published the relation of the types of tumor, the proliferation rate, and MYCN gene amplification with a number of autophagic vacuoles (Samardzija G et al, 2016). We are now further analyzing various types of autophagy in neuroblastoma. Together with that, we are investigatingthe role of autophagy in metabolic diseases.
What is the aim of the project?
The aim of the project is to investigate relation of various types of autophagy and the type of peripheral neuroblastic tumors by the use of electron microscopy. In metabolic diseases we are investigating the impact of the different therapeutic modalities on the presence of various types of autophagic vacuoles.
What techniques and methods are used?
Preparation of the cells and tissues for electron microscopy (fixation, embedding in resins, cutting of semithin and ultrafine sections), electron microscopic evaluation and morphometric analysis of autophagic vacuoles will be done in this project.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student is expected to do morphometric analysis on electron micrographs. The student will have the opportunity to perform some of the steps in preparation of the samples for electron microscopy under the supervision, including preparation of fixatives and embedding in resins. Student will be offered to observe the cutting procedures and preparation of ultrafine sections. Ultrastructural analysis of features of tumor tissue samples on electron microscope will be done with the supervision.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Theoretical explenation of both preaparation techniques for electron microscopy as well as the explenation of the required morphometric methods will be provided.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No prior skills are required Subjects passed: histology (official course or as a part of other subjects)
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
- Samardzija G; Stevovic TK; Djuricic S; Djokic D; Djurisic M; Ciric D; Martinovic T; Bumbasirevic V; Vujic D. Aggressive human neuroblastomas show a massive increase in the numbers of autophagic vacuoles and damaged mitochondria.UltrastructPathol. 2016;40(5):240-8.
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