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Evaluation of the effect of different factors on extracting forensic evidence in rape. (Assuit University)
Egypt (IFMSA-Egypt) - Assiut University, Assiut
Department of Forensic Medicine, Faculty of Medicine, Assiut University, Assiut, Egypt.
Prof. Dr. Wafaa Abdelmounem
Dr. Noha Ismail
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
In many cases of rape, the biological materials are recovered after examination of the victim or may be submitted after long time for DNA analysis or may be lying pending for DNA analysis. Therefore, the present work was taken up to study the effect of storage conditions and yield of DNA from different textures of cloth for seminal stains, the biological material forwarded in rape cases. The seminal stains recovered on fabrics are one of the most important evidence in rape cases. Stained clothing’s, undergarments and swab’s on fabric material taken from vagina and surrounding regions, are submitted for DNA Fingerprinting test to compare it with the blood sample of the accused person.
What is the aim of the project?
The aim of this work is to evaluate the effect of different factors on extraction of blood and seminal stain DNA from clothes.
What techniques and methods are used?
This study includes DNA extraction, PCR detection of antigens, electrophoresis
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- If the project is clinical
- the student will be allowed to work with patients
- The tasks of the student will be performed on his/her own
What are the tasks expected to be accomplished by the student?
Observation of extraction of DNA of blood and seminal stains from clothes and exposing them it different environmental factors such as cold, heat burn, alkali, corrosives and observing their effect on The DNA profiling visualization by spectrophometry, bioanalyzer, and finally profiling by PCR, also data analysis and results comprehension are expected to be done under supervision of the professor first, then done on the student’s own, and the professor will review and discuss the results with them at the end.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Lectures conecring the topic; presentation of the clinical skills used.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - The student will prepare a poster - The student will prepare a presentation - The student will prepare an abstract
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
It's preferred if the student have studied Forensic Medicine.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- 1-Bomjen G; Raina A; Sulaiman IM; Hasnain SE; Dogra TD (1996) :Effect of storage of blood samples on DNA yield; quality and fingerprinting: a forensic approach; Indian J Exp Biol. 34(4):384-6.
- 2-Beckett; S.M.; Laughton; S.J.; Pozza; L.D.; McCowage; G.B.; Marshall; G.; Cohn; R.J.; et al. (2008): Buccal swabs and treated cards: methodological considerations for molecular epidemiologic studies examining pediatric populations. Am. J. Epid.; ; 167 (10); 1260-1267-
- 3- Jeffreys AJ; Wilson V; Thein SL (1985): Individual-specific 'fingerprints' of human DNA. Nature 316; 76-79
- 4-Kathryn M. Turman (2001): Understanding DNA evidence: a guide for victim service providers; OVC bulletin ;p:1-11.
- 5-Kannika Sutthapodjanarux; Nathinee Panvisavas; and Nopadol Chaikum. (2009): the 4th CIFS academic day ; accessed on line in june;2014.
- 6-Karolyn L. Tontarski; M.S.; Kyle A. Hoskins; B.S.; Tani G. Watkins; B.A.; Leanora Brun- Conti; B.S.; and Amy L. Michaud; B.S. (2009): Chemical enhancement techniques of bloodstain patterns and DNA recovery after fire exposure. Forensic Sci January 2009; Vol. 54; No. 1 ? J;p;37-47.
- 7- Raina A; Dogra TD (2003) :Methods of collection; Preservation and Forwarding of Biological Material for DNA Fingerprinting. Ind Internet J Forensic Med Toxicol 1(2);2003; www.icfmt.org
- 8-Raina A; Pramanik P; Dogra TD;(2005): effect of storage conditions of seminal stains on diffrent textures of cloths in relation to DNA yield. IIJFMT 3(2).
- 9-Smith; L.M.; Burgoyne; L.A.(2004): Collecting; archiving and processing DNA from wildlife samples using FTA databasing paper. B.M.C. E. col.; 2004; 4; 4:1-11.
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