Projects
Name
Identification of circadian rhythm related proteins expression changes in colorectal Carcinoma
University
Comenius University
Domain
Pathology
Departement
Department of Pathology, Faculty of Medicine Comenius University
Head
prof. Ludovit Danihel, MD. PhD.
Tutor
prof. Pavel Babal, MD. PhD
Languages
English (required) , Slovak, Czech (accepted)
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes Yes No No No No
Type of Research Project
- Basic science
What is the background of the project?
Certain proteins are expressed in different cells in a periodical rhythmical sinusoidal manner. A group of proteins have periodical increases/decreases of expression corresponding to the day-night rhythm. By means of immunohistochemistry we will map changes in expression of circadian rhythm-related proteins in colorectal carcinoma in comparison to normal intestinal mucosa.
What is the aim of the project?
The aim of the project is evaluation of changes in expression of circadian rhythm-related proteins in colorectal carcinoma in comparison to normal intestinal mucosa, assesment of their prognostic and predictive value.
What techniques and methods are used?
Used techniques: Routine histology for basic diagnostics ov the disease; histochemistry with identification of mucins production by the tissue (PAS, Alcian blue); immunohistochemistry with different antibodies against circadian rhythm-related proteins; morphometry using computerized two-dimensional image analysis for evaluation of particular protein expression.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
will to work in a laboratory
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
yes
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No special skills are required. Subjects passed: Pathology
Are there any legal limitations in the student’s involvement
No
Hours
8
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
Articles
- Davis S; Mirick DK; Stevens RG. Night shift work; light at night; and risk of breast cancer. J Natl Cancer Inst. 2001 Oct 17;93(20):1557-62.
- Shende VR; Kim SM; Neuendorff N; Earnest DJ. MicroRNAs function as cis- and trans-acting modulators of peripheral circadian clocks. FEBS Lett. 2014 Aug 25;588(17):3015-22. doi: 10.1016/j.febslet.2014.05.058.
- Innominato PF; Palesh O; Dhabhar FS; L