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Molecular determinants of autophagy modulating reagents for cancer therapy
Tokyo Medical University
Masaki Hiramoto, Naoharu Takano
Type of Research Project
- Basic science
What is the background of the project?
Autophagy-lysosome system and ubiquitin-proteasome system are two major protein degradation systems in eukaryotic cells. These systems are highly regulated, and are responsible for maintenance of protein homeostasis and adaptation to environmental changes. In cancer cells, autophagy-mediated recycling is indispensable to maintain energy homeostasis to meet the elevated metabolic demand of growth and proliferation. Thus, autophagy inhibition may be beneficial for cancer therapy. We and other groups have reported that the macrolide antibiotics and tyrosine kinase inhibitors, which have been used in clinical treatment, modulated the autophagy flux.
What is the aim of the project?
Identification and characterization of cellular targets for macrolide antibiotics and tyrosine kinase inhibitors in order to develop the new cancer therapy targeting autophagy-lysosome system
What techniques and methods are used?
Cell culture, Morphological observation, Cell proliferation assay, Immunoblotting, Polymerase chain reaction (PCR), Gene recombination etc.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
Students need to perform the experiments, which include basic methods in molecular biology (e.g. cell culture, polymerase chain reaction (PCR), real-time PCR, western blotting) and specific assays for autophagy (such as autophagy flux asssay and analysis of cellular morphology), analyze these data, read the papers, and discuss the concepts, methods and results of the research. Students are also expeted to attend the Journal club and Data club of our laboratory.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Discussion about the concepts, methods and results of the research.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Students need to complete the lectures on biochemistry and molecular biology at the university. There is no legal limitation. Scientific report is not demanded after the exchange. Also, students need to pass their biochemistry and molecular biology.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields - Dental medicine students (IADS members)
- doi: 10.3892/ijo.2016.3673.
- doi: 10.3892/ijo.2015.3237.
- doi: 10.1016/j.bbrc.2015.03.162.
- doi: 10.3892/ijo.2014.2773.
- doi: 10.1016/j.bbrc.2013.06.032.
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