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Evaluation of choroid on diabetic retinopathy
Portugal (PorMSIC) - University of Coimbra, Coimbra
IBILI Institute for Biomedical Imaging and Life Sciences
English or Portuguese
Type of Research Project
- Basic science
What is the background of the project?
Diabetic retinopathy is the leading cause of visual impairment in working-age adults worldwide. The disease is characterized by microaneurysms, capillary nonperfusion, and ischemia within the retina, ultimately leading to neovascularization and/ormacular edema, both of which can severely compromise visual function. In diabetes retinopathy, there are alterations present in the choroid that may be relevant for the onset and progression of diabetic retinopathy.
What is the aim of the project?
Evaluation of morphology and the number of immune cells present in the choroid of a rat model of type 1 diabetes.
What techniques and methods are used?
Optical coherence tomography, eye sectioning in a cryostat, immunohistochemistry, fluorescence microscopy, and western blot.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
What are the tasks expected to be accomplished by the student?
The techniques performed by the student will depend on his knowledge and experience with basic techniques in a cell biology laboratory and animal handling. He will be able to observe optical coherence tomography and eye sectioning in a cryostat and he will peform immunohistochemistry, fluorescence microscopy and western blot after practising it sometimes under supervision.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Campos A; Campos EJ; Martins J; Ambrosio AF; and Silva R. (2016) Viewing the Choroid: Where We Stand; Challenges and Contradictions in Diabetic Retinopathy and Diabetic Macular Oedema. Acta Ophthalmol. Accepted for publication.
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