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Immunology and Genetics Studies in Children and Adolescent with Type 1 Diabetes
Thailand (IFMSA-Thailand) - Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok
Prof. Dr. Tararaj Dharakul
Dr. Watip Tangjittipokin
Type of Research Project
- Basic science
What is the background of the project?
Diabetes is a chronic disease and a major global public health problem. Currently, the prevalence of diabetes in increasing worldwide, including in Thailand. Diabetes in a major cause of illness, premature death, and other complications such as eye, renal, cardiac, and neural diseases. Studying the pathogenesis of the disease may reduce risk factors and provide early treatment. Type 1 diabetes is an autoimmune and multifactorial disease with a strong genetic control. The project aims to investigate the distribution of human leukocyte antigen (HLA) allele and non-HLA genes in Type 1 diabetes comparing with healthy controls. Techniques used are DNA extraction, Polymerase chain reaction (PCR), cloning, protein purification, cell culture, Western blot analysis, Real time PCR, Next generation sequencing, and Sanger sequencing.
What is the aim of the project?
To investigate the distribution of human leukocyte antigen (HLA) allele and non-HLA genes in Type 1 diabetes comparing with healthy control. With this information, analysis for underlying mechanism of type 1 diabetes can be conducted further. Moreover, genetics screening for prevention and early detection may be at least established.
What techniques and methods are used?
Deoxyribonucleic (DNA) extraction, Polymerase chain reaction (PCR), Cloning, Protein purification, Cell culture, Western blot analysis, Real time PCR, Next generation sequencing, Sanger sequencing
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
The student should be able to explain the overall project and objective of some experiments which are available at that time in the ongoing project. To illustrate, during your exchange you may have a chance to experience some molecular technique such as Deoxyribonucleic (DNA) extraction, Polymerase chain reaction (PCR) and Western blot. The student is expected to have responsibilities and laboratory duties; such as checking some instrumental maintenance daily or weekly. The student will join the academic activities such as lab meetings, journal clubs and seminars.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields - Dental medicine students (IADS members)
- Sujjitjoon J; Kooptiwut S; Chongjaroen N; et al. PAX4 R192H and P321H polymorphisms in type 2 diabetes and their functional defects. Journal of Human Genetics. 2016;61(11):943-949.
- Sujjitjoon J; Kooptiwut S; Chongjaroen N; et al. Aberrant mRNA splicing of paired box 4 (PAX4) IVS7-1G>A mutation causing maturity-onset diabetes of the young; type 9. Acta Diabetologica. 2015;53(2):205-216
- Plengvidhya N; Chanprasert; Tangjittipokin W; et al. Detection of CAPN10 copy number variation in Thai patients with type 2 diabetes by denaturing high performance liquid chromatography and real-time quantitative polymerase chain reaction. Journal of Diabetes Investigation. 2015;6(6):632-639.
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