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Molecular mechanisms of cell migration and invasion: developing a new strategy to impair tumor progression
Portugal ( PorMSIC) - Nova University of Lisbon, Lisbon
Membrane Traffic in Infection and Disease Lab, CEDOC, NOVA Medical School
Duarte Barral and Cristina Casalou
Type of Research Project
- Basic science
What is the background of the project?
Migration and invasion of cancer cells are essential for the formation of metastases, yet the mechanisms involved and the role of actin remodeling in this process are not fully understood. We have established a new role for the small G protein Arl13b and the myosin NMIIA in cell migration in non-pathological conditions (Casalou et al., 2014). Moreover, our preliminary studies show that Arl13b regulates breast and colon cancer cell migration and invasion in vitro and that Arl13b silencing reduces tumor growth in vivo. We now aim to establish whether NMII plays a similar role to Arl13b in cancer cell migration an d invasion and if NMII can be used as a biomarker of breast cancer progression.
What is the aim of the project?
Our specific aims are: (1) to assess the role of NMIIA and B in cancer cell migration and invasion; and (2) to quantify NMIIA and B protein levels in breast tissue samples
What techniques and methods are used?
Tissue culture, western-blot, real-time quantitative polymerase chain reaction, invasion assays and migration assays
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will participate in all the steps of the experimental process and is expected to learn how to design and perform on his own the following techniques: tissue culture, western-blot and real-time quantitative polymerase chain reaction and under supervisition invasion assays and migration assays and how to interpret experimental results and understand their meaning.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The student will have to read ahead of time the literature provided.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
At least 2 years after high school.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Casalou C.; Seixas C. Portelinha A.; Barros M.; Pintado P.; Ramalho J.S.; Lopes S.S.; Barral D.C. (2014) Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration. J. Cell Sci. Jun 15;127(Pt 12):2709-22. doi: 10.1242/jcs.143446. Epub 2014 Apr 28.
- Heissler S.M.; Manstein D.J. (2013) Nonmuscle myosin-2: mix and match. Cell Mol Life Sci. Jan;70(1):1-21. doi: 10.1007/s00018-012- 1002-9. Epub 2012 May 8.
- Barral D.C.; Garg S.; Casalou C.; Watts G.; Ramalho J.S.; Hsu V.W.; Brenner M.B. (2012) Arl13b regulates endocytic recycling traffic. Proc Natl Acad Sci U S A. Dec 26;109(52):21354-9. doi: 10.1073/pnas.1218272110. Epub 2012 Dec 7.
- Vicente-Manzanares M.; Ma X.; Adelstein R.S.; Horwitz A.R. (2009) Non-muscle myosin II takes centre stage in cell adhesion and migration. Nat Rev Mol Cell Biol. Nov;10(11):778-90. doi: 10.1038/nrm2786.
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