Projects
Name
Role of inflammatory mediators in the recruitment and polarization of tumor-associated macrophages and neutrophils
University
Universita degli Studi di Napoli 'Federico II'
Domain
Immunology
Departement
Laboratory of Leukocyte Biology, Department of Immunology, Via Manzoni 113, 20089, Rozzano, Milano, Italy
Head
Professor Alberto Mantovani
Tutor
Raffaella Bonecchi (Associate Professor, PhD); Matteo Massara (PhD student), Valeria Mollica Poeta (PhD student); E-mail: raffaella.bonecchi@humanitasresearch.it; matteo.massara@humanitasresearch.it; Valeria.Mollica_Poeta@humanitasresearch.it Phone: 0039
Languages
English, Italian
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No No No No No No No
Type of Research Project
- Basic science
What is the background of the project?
Both macrophages and neutrophils are key elements of the cancer-related inflammation. The project will investigate mechanisms that induce myeloid cell release from the bone marrow and tumor-derived molecules that affect myeloid cell differentiation inducing a phenotype that supports tumor growth, inducing immunosuppression, angiogenesis and metastasis.
What is the aim of the project?
identify new mechanisms to be targeted to promote anti tumoral immune response.
What techniques and methods are used?
In order to evaluate the role and the phenotype of myeloid cells during tumor growth 1. In vivo tumour models will be performed with orthotopical injection of tumor cells in mice. 2. Tumor growth will be followed measuring tumor uptake and dimensions with an electronic caliper and by in vivo imaging. 3. Explanted tumors will be disaggregated and studied for their leukocyte infiltrate by Fluorescence-Activated Cell Sorter (FACS) analysis 4. Explanted tumors will be frozen or paraffin included and studied by immunohistochemistry. 5. Blood will be studied for circulating leukocyte frequency and phenotype by FACS analysis. 6. Serum will be analysed for cytokine content by enzyme-linked immunosorbent assay (ELISA). 7. Statistical analysis of the obtained data
What is the role of the student?
- The student will mainly observe
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
-
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
0
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
the student should have passed the Immunology exam before the exchange
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
Articles
- Bonavita O; Massara M; Bonecchi R. Chemokine regulation of neutrophil function in tumors. Cytokine Growth Factor Rev. 2016
- Savino B; Caronni N; Anselmo A; Pasqualini F; Borroni EM; Basso G; Celesti G; Laghi L; Tourlaki A; Boneschi V; Brambilla L; Nebuloni M; Vago G; Mantovani A; Locati M; Bonecchi R. ERK-dependent downregulation of the atypical chemokine receptor D6 drives tumor aggressiveness in Kaposi sarcoma. Cancer Immunol Res. 2014 Jul;2(7):679-89.