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Development of depression and changes in activation of hypothalamic-pituitary-adrenal (HPA) axis in Wistar rat
Bulgaria (AMSB) - Medical University of Pleven, Pleven
Associated professor Milena Atanasova
Associated professor Milena Atanasova
Type of Research Project
- Basic science
What is the background of the project?
Experimental model of AD provoked by intracerebroventricular injection of streptozotocin (STZ-ICV) leads to depressive type behavior and biochemical and morphological alterations in Wistar rats. Verification in comorbid depression in STZ-ICV model of AD is suitable for future screening of potential drugs positively affecting the development of depression in this disease.
What is the aim of the project?
The aim of this project is to investigate the effects of the selective melatoninergic agonist agomelatine on the development of depressive behavior in a model of Alzheimer's disease in male Wistar rats.
What techniques and methods are used?
Experimental groups of animals: 45 male Wistar rats divided into 3 groups: 1) Sham - saline-injected ICV (n = 15); 2) AD - treated with STZ (n = 15). 3) AD ago - treated with STZ and agomelatine (n = 15). Behavioral methods to analyze the effects of experimentally induced AD on total motor activity, depression and anxiety. • Methods for studying the state of motion and research activity and anxiety (open field; elevated plus maze) • Novelty-suppressed feeding test, "Saccharin preference test", "splash test", "Forced swimming test"; • Anxiety investigation methods - "light-dark"; "Elevated plus maze"; "Open field". Biochemical methods Investigation of amyloid-beta precursor protein (ELISA) and phosphorylated tau-protein (Immunoblot) in frontal core and hippocampus. Testing inflammation in brain structures by measuring TNF alpha. Statistics of the results – SPSS, v. 19
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
The students will be able to participate in selection and arrangement of the investigated sera and tissue for ELISA test for TNF alpha and beta-amyloid peptide. They will participate in the performing of ELISA and immunoblot for investigation of Tau protein. Later the statistical analysis of the results will be performed.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Theoretical and practical abilities with ELISA method. The students should have some practice with preparation of buffers, with performing of commercial ELISA (to be able to make solutions, to work with automatic pipettes). There are no legal limitations.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Students in biomedical fields
- 1. Tchekalarova J; Nenchovska Z; Atanasova D; Atanasova M; Kortenska L; Stefanova M; Alova L; Lazarov N. Consequences of long-term treatment with agomelatine on depressive-like behavior and neurobiological abnormalities in pinealectomized rats. Behavioural Brain Research 302 (2016) 11?28;
- 2. Tchekalarova J; Ivanova N; Pechlivanova D; Illieva K; Atanasova M. Strain-dependent effects of chronically infused losartan against kainic acid induced seizures; oxidative stress and heat shock protein 72 expression. Cell Mol Neurobol; 2014 Jan;34(1):133-42. doi: 10.1007/s10571-013-9994-8. Epub 2013 Oct 22.
- 3. Atanasova M; Z Petkova; D Pechlivanova; P Dragomirova; A Blazhev; J Tchekalarova. Strain-dependent effects of long-term treatment with melatonin on kainic acid-induced status epilepticus; oxidative stress and the expression of heat shock proteins. Pharmacology; Biochemistry and Behavior; 111; (2013); 44-50
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