Projects
Name
Investigation of skin microbiome in atopic dermatitis
University
Institute of Medical Biology, A* STAR Singapore
Domain
Dermatology
Departement
Institute of Medical Biology
Head
Dr Birgit Lane
Tutor
Dr John Common, Dr Angeline Tay
Languages
English
Duration
8 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No Yes Yes Yes Yes No No No No
Type of Research Project
- Basic science
What is the background of the project?
This project will investigate changes in microbiome diversity in subjects with Filaggrin (FLG) -null mutations vs controls. The student will likely prepare sequencing libraries of microbiome samples for next generation sequencing, perform FLG-null mutation sequencing and work with in vitro models that are being developed to understand the mechanism by which certain microbes alter the skin barrier and cause disease. Aims: - Better understand how the skin microbiome interacts with the host Technique used: a full range of lab techniques necessary for completion of the project including molecular biology, cell culture, histology techniques. Eg. Whole metagenomic analysis, Bacterial challenge assays using keratinocytes and monocyte-derived dendritic cells, comparison of skin microbiome signatures
What is the aim of the project?
What techniques and methods are used?
What is the role of the student?
What are the tasks expected to be accomplished by the student?
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
1
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- Student will be highly involved to take an active role in the practical aspect of the project and results analysis. They will be mentored in the lab by a Postdoc researcher. After student has shown competence they can be performed unsupervised.
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
2nd Year University degree completed; - knowledge of skin biology, knowledge of microbiome; - Students should also be motivated and previous experience with biology would come in useful in this project.
Are there any legal limitations in the student’s involvement

Hours
7
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
Articles
- Whole metagenome profiling reveals skin microbiome-dependent susceptibility to atopic dermatitis flare. Chng KR; Tay AS; Li C; Ng AH; Wang J; Suri BK; Matta SA; McGovern N; Janela B; Wong XF; Sio YY; Au BV; Wilm A; De Sessions PF; Lim TC; Tang MB; Ginhoux F; Connolly JE; Lane EB; Chew FT; Common JE; Nagarajan N. Nat Microbiol. 2016 Jul 11;1(9):16106.