Studies on 13C Isotopic Fractionation from Cancer Patients Samples using Isotope-Ratio Mass Spectroscopy (IRMS)
Brazil (DENEM) - Pontificia Universidade Catolica do Rio Grande do Sul (PUC-RS), Porto Alegre-RS
Institute of Biomedical Research - Drug Discovery Lab
Paulo Marcio Condessa Pitrez
Dyeison Antonow
English, Portuguese, Spanish
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No Yes Yes Yes Yes Yes Yes No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
There is a compilation of molecular elements that support the hypothesis of detecting isotopic fractionation in tumors. The best evidence can be attributed to cancer metabolism processes, which differs from normal tissues for both sugars and lipids, the former has already been exploited in diagnostic techniques (PET-Scan). Evaluate whether the IRMS technique (mass spectrometry isotope ratio) has the potential to detected any isotopic fractionation in tumors. This could set the basis for screening tests that could be used to monitor disease at a populational level.
What is the aim of the project?
The research project has two objectives: a) to determine if a tumor biopsy incorporates different fractions of 13C in its structure compared to adjacent healthy tissues; and b) to determine whether cancer-diagnosed patients have different fractions of 13C in their breath and urine samples compared to healthy volunteers.
What techniques and methods are used?
For that, will be used Surgical Oncology, Pathology and Isotope-Ratio Mass Spectroscopy (IRMS) during the entire duration of the exchange program.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
For that, will be used Surgical Oncology, Pathology and Isotope-Ratio Mass Spectroscopy (IRMS) during the entire duration of the exchange program.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Preferably a second year medical college but not mandatory. A variety of skills and, most importantly, a multidisciplinary approach to perform highly innovative research on cancer diagnosis. The Institutions (PUCRS) may ask for a Non-Disclosure Agreement and/or and assignment-of-inventions agreement to protect PUCRS intellectual property. A report will be required from the student directly to Prof. Dyeison Antonow
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
- McCue MD; Welch KC Jr. (13)C-Breath testing in animals: theory; applications; and future directions. J Comp Physiol B. 2016 Apr;186(3):265-85.
- Il-Young Kim; Sang-Hoon Suh; In-Kyu Lee; and Robert R Wolfe. Applications of stable; nonradioactive isotope tracers in in vivo human metabolic research. Exp Mol Med. 2016 Jan; 48(1): e203.
- Braden B. Methods and functions: Breath tests. Best Pract Res Clin Gastroenterol. 2009;23(3):337-52.