Research of multilayer polyelectrolyte microcapsules uptake influence on adhesion and migration activity of mesenchymal stem cells.
Russian Federation (HCCM Russia) - St. Petersburg State Medical Academy, St. Petersburg
Laboratory of transplantology and molecular hematology, R.M. Gorbacheva Memorial Institute of Pediatric Oncology and Transplantation.
Prof. Vladislav V. Sergeev, MD
Dr. Kirill V. Lepik, MD
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
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Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Mesenchymal stem cells (MSCs) are self-renewing, multipotent progenitor cells with multilineage differentiation potential are one of the most widely accepted cell population in cell therapy approaches with more than 500 attempted clinical trials. The reason for such great interest is their relatively easy accessibility, convenient, well established and reproducible expansion protocols, and unique biological properties. There is growing demand for enhancing required biological properties, and to improve or to control MSC tropism after administration. To achieve this purposes, the microcapsules technology is sought to be encouraging approach to alter MSCs functional delivery. Multilayer polyelectrolyte microcapsules being impregnated into stem cells show great promise as diagnostic, therapeutic and investigational tool proving externally controlled drug and cell delivery .
What is the aim of the project?
The aim of this work is to explore the influence of microcapsules uptake on adhesion and migration activity of mesenchymal stem cells.
What techniques and methods are used?
Methods of cultural studies: mesenchymal stem cells separaton, seeding, passaging. Flow cytometry, fluorescence microscopy, confocal laser scanning microscopy. Non-parametric statistics using IBM SPSS and R software –
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will be directly involved in the project at all stages of the study: harvesting bone marrow, bone marrow separation, initiation and passaging the MSC culture, synthesis of the microcapsules, fluorescence and confocal microscopy, flow cytometry results analysis, statistical analysis.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, participating student may visit seminars and lectures delivered in the R.M. Gorbacheva Memorial Institute of Pediatric Oncology and Transplantation (1-5 per month, particular lectures titles are depending on the month). Also the center tour, introduction seminar will be performed.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
ACCEPTED ONLY students from the 3d year !!! Knowledge of the basic principles of work with human/animal cells. Final test/exam in medical biology, heamotology and oncology should be passed.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- James A Ankrum et al Engineering cells with intracellular agent?loaded microparticles to control cell phenotype Nature Protocols 9; 233?245 (2014) doi:10.1038/nprot.2014.002