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Functional analysis of cardiac ion channels under physiological and pathophysiological conditions.
Japan (IFMSA-Japan) - Shiga medical university, Shiga
Mariko Omatsu-Kanbe, Wei-Guang Ding, Futoshi Toyoda
Type of Research Project
- Basic science
What is the background of the project?
Description: We have been investigating the function of ion channels and transporters in the heart to clarify their roles in cardiac function by using electrophysiological, pharmacological and molecular biological methods. In addition, we have been conducting experiments to elucidate molecular basis for the regulation of ion channels by neurotransmitters, hormones, intracellular molecules and exogenous drugs that affect cardiac electrical activity. Specifically, we are currently conducting several research projects, such as i) Regulation of cardiac ion channels by intracellular signaling cascades; ii) Ionic mechanisms underlying sinoatrial (SA) node automaticity; iii) Molecular mechanisms underlying hereditary arrhythmias such as Long QT syndrome; iv) Cardioprotective action of volatile anesthetics. Aim: We hope to provide new information to understand the physiological and pathophysiological roles of cardiac ion channels and to develop novel therapeutic strategies to treat various cardiac disorders. Used Techniques: Electrophysiological (patch-clamp, Ca2+ imaging), molecular biological (site-directed mutagenesis and RT-PCR) and computer simulation methods.
What is the aim of the project?
We hope to provide new information to understand the physiological and pathophysiological roles of cardiac ion channels and to develop novel therapeutic strategies to treat various cardiac disorders.
What techniques and methods are used?
Electrophysiological (patch-clamp, Ca2+ imaging), molecular biological (site-directed mutagenesis and RT-PCR) and computer simulation methods.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
To participate in our research project and discuss the experimental data with members of the project team. The student can obtain the experience of conducting the electrophysiological, pharmacological and molecular biological experiments, such as i) patch-clamp experiments and intracellular Ca2+ imaging using confocal microscope on isolated cardiac myocytes from mouse, rat and guinea-pig heart, ii) transfection of ion channel gene into cultured cells, and iii) site-directed mutagenesis in ion channel gene.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
If necessary, we will provide theoretical teaching seminars to the vising students.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
In general, special skills are not required. There are no any legal limitatons in the student
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
- Bai JY; Ding WG; Kojima A; Seto T; Matsuura H. (2015) Putative binding sites for arachidonic acid on the human cardiac Kv1.5 channel. Br J Pharmacol 172:5281-92.
- Kojima A; Ito Y; Ding WG; Kitagawa H; Matsuura H. (2015) Interaction of propofol with voltage-gated human Kv1.5 channel through specific amino acids within the pore region. Eur J Pharmacol 764:622-32.
- Xie Y; Ding WG; Matsuura H. (2015) Ca2+/calmodulin potentiates IKs in sinoatrial node cells by activating Ca2+/calmodulin-dependent protein kinase II. Pfl
- Kojima A; Ito Y; Kitagawa H; Matsuura H. Ionic mechanisms underlying the negative chronotropic action of propofol on sinoatrial node automaticity in guinea pig heart. Br J Pharmacol 2015;172:799-814.
- Kojima A; Ito Y; Kitagawa H; Matsuura H; Nosaka S. Direct negative chronotropic action of desflurane on sinoatrial node pacemaker activity in the guinea pig heart. Anesthesiology 2014;120:1400-13.
- Kojima A; Kitagawa H; Omatsu-Kanbe M; Matsuura H; Nosaka S. Sevoflurane protects ventricular myocytes against oxidative stress-induced cellular Ca2+ overload and hypercontracture. Anesthesiology 2013;119:606-20.
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