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Estrogen and tumor progression
Italy (SISM) - University of Milan, Milan
Pharmacology and Biomolecular Sciences
Prof. Adriana Maggi
Type of Research Project
- Basic science
What is the background of the project?
Estrogens as sex steroids are well known for their action at the molecular level and in the regulation of reproductive function, but little is known with regard to their involvement in the activity of organs other than reproductive. Also through the creation of innovative models for the study of estrogen action by means of molecular imaging we and other demonstrated that these hormones have a major impact on the functioning of the cardiovascular, skeletal, nervous and immune systems. Yet, at present we have very little insights on the mechanisms underlying estrogen action in non-reproductive tissues. The goal of our investigation is to fully understand estrogen activities in non-reproductive tissues to identify drugs able to overcome estrogen deficiency in case of gonadal dysfunction and aging. See http://www.disfeb.unimi.it/ecm/home/ricerca/laboratori-ricerca/laboratorio-di-biotecnologie-farmacologiche
What is the aim of the project?
Genomic analysis aimed at identifying early signalling in malignant transformation and its translation to clinics.
What techniques and methods are used?
Mouse genetics, molecular and cell biology techniques, molecular imaging. In particular: - Molecular cloning technologies (all the basic technologies needed for DNA cloning) - Biochemical assays for enzymatic activities (deacetylases, acetylases, ciclooxygenases etc) - Analysis of gene expression (real time Polymerase Chain Reaction, Northern blot, western blot, run on, etc) and of DNA (Polymerase Chain Reaction, genotyping, Restriction Fragment Lenght Polymorphism analysis, Southern blot etc) - Genomic analysis (mainly transcriptomics) - Transcriptome analysis and of bioinformatic analysis of existing genomic datasets - Cell biology techniques including transfections, knock down , immunocytochemistry - Bioluminescence imaging of proliferation, inflammatory reactions, oxidative stress and estrogen receptor activation during carcinogenic progression in genetic and chemical models of breast tumorigenesis in the mouse. - Ex vivo analysis of tissues including histopathology and immunohistochemistry of tumor specific genes.
What is the role of the student?
- The student will mainly observe
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No particular skills required. The student is needed to have passed the following subjects: basic knowledge of biochemistry and cell biology.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
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