Study of Folicular T helper responses in volunteers vaccinates for Dengue using an attenuated recombinant vaccine
Brazil (DENEM) - Pontificia Universidade Catolica do Rio Grande do Sul (PUC-RS), Porto Alegre-RS
Institute of Biomedical Research, PUCRS
Paulo Marcio Pitrez, MD PhD
Cristina Bonorino, PhD
English , Portuguese, Spanish.
4 weeks
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Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Dengue is a major disease afflicting tropical countries. Humans can be infected with 4 major serotypes/strain of the virus, and in some cases develop a life-threatening hemorrhagic disease. One vaccine by Sanofi has been licensed but does not induce protection to all serotypes. We are conducting a vaccine trial using an attenuated vaccine of all serotypes developed by the NIH. Because neutralizing antibodies are an important aspect of protection we will analyze the response of follicular T helper cells that are determinant for neutralizing antibody formation.
What is the aim of the project?
What is the aim of the project? To correlate antibody responses, protection, and measurable T follicular helper responses in vaccinated volunteers.
What techniques and methods are used?
What techniques and methods are used? Cell analysis by flow cytometry at different points after vaccination.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
What techniques and methods are used? Cell analysis by flow cytometry at different points after vaccination.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Dedication; good interpersonal relationships; lab background is always good; good writing is a plus. Subjects passed: immunology.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
- The human immune response to Dengue virus is dominated by highly cross-reactive antibodies endowed with neutralizing and enhancing activity.
- The live attenuated dengue vaccine TV003 elicits complete protection against dengue in a human challenge model.