Projects
Name
Analysis of genetic alterations in human malignancies
University
Germany (bvmd) - Medizinische Hochschule Hannover, Hannover
Domain
Genetics
Departement
Department of Human Genetics, OE 6300 Hannover Medical School Carl-Neubergstr. 1, 30625 Hannover
Head
Prof. Dr. med. Brigitte Schlegelberger
Tutor
Prof. Dr. Doris Steinemann, Dr. Britta Skawran, PD, Dr. Gudrun Goehring
Languages
English, German
Duration
8 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No Yes Yes Yes Yes No No No No No No
Type of Research Project
- Basic science
What is the background of the project?
The main focus of research lies within the investigation of a large variety of genetically caused diseases. For the identification of novel genetic markers for individualized medicine we use Array-CGH (Comparative genomic hybridization), panel sequencing, NGS (Next generation sequencing of exome, transcriptome) and metabolom analysis. Within the area of epigenetics we analyze histone modifications and their impact on cancer development and progression at the example of hepatocellular carcinoma.
What is the aim of the project?
The aim of this project is to learn different techniques of the identification of genetic alterations that can lead to cancer and different syndromes. This will possibly allow for the identification of novel genetic markers for individualized medicine and genetic counseling.
What techniques and methods are used?
PCR (polymerase chain reaction) techniques, cultivation, insight into different methods of sequence analysis, e.g. array-CGH (Comparative genomic bridization), FISH (Fluorescence in situ hybridization), panel sequencing, chromatin immunoprecipitation.
What is the role of the student?
What are the tasks expected to be accomplished by the student?
-
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
1
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
An eligible candiadte has profound knowledge of human genetics and genetic malignancies, and in particular proven hands on training with basic laboratory skills (e.g. DNA/RNA extraction, PCR, microscopy). Previous experience with: NGS (preferred) (exome, transcriptome) Considering the amount of time that is required to properly teach the used techniques a stay shorter than 8 weeks is not applicable. After the project you will be able to independently use the learned techniques
Are there any legal limitations in the student’s involvement
No
Hours
9
Type of students accepted
This project accepts:
- Medical students
- Pre-Medical students from the American-British system
Articles
- The heteromeric transcription factor GABP activates the ITGAM/CD11b promoter and induces myeloid differentiation. Ripperger T; 2015; Biochim Biophys Acta
- Expression of the ETS transcription factor GABP