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Inflammation-induced bone destruction in vitro and in vivo: from mechanistic investigation to therapeutic potentials
Taiwan (FMS-Taiwan) - National Defense Medicine Center, Taipei
School of Dentistry, Tri-Service General Hospital and National Defense Medical Center
Ren-Yeong Huan, DDS, Ph.D
Ren-Yeong Huang, DDS, Ph.D; Wan-Chien Cheng, DDS, Ph.D; Tian-Syuan Lin, MS (Research assistant); An-Jui Liu, (student , master program), Fang Yi Tseng, DDS, (student , master program), Po-Yan Hsiao, MS (student , Ph.D program)
Type of Research Project
- Basic science
What is the background of the project?
Periodontal disease is the most frequent cause of tooth loss in humans and is characterized by destruction of the attachment apparatus, and loss of alveolar bone. Periodontal inflammatory process is initiated by bacteria colonization, dysregulation of immune response leading to infiltration of inflammatory cells, excess production of cytokines, the elaboration of lytic enzymes and the activation of osteoclasts which responsible for loss of connective tissue and alveolar bone. Osteoclasts are multinucleated giant cells formed by fusion mononuclear progenitors of monocytes/macrophage lineage through a differentiation process that is mainly modulated by receptor Activator for Nuclear Factor Kappa B Ligand (RANKL) and macrophage colony stimulating factor (M-CSF). Excessive osteoclast formation has been observed in various bone diseases. Therefore, suppressing RANKL-induced osteoclast differentiation related signaling pathways is a therapeutic target for preventing osteoclast associated diseases, including rheumatoid arthritis, periodontal disease, and osteoporosis.
What is the aim of the project?
The aims of this project are (1) to investigate the mechanism by which periodontal pathogen infection induces an inflammatory bone destruction in vitro and in vivo; (2) to investigate the effect of candidate therapeutic drug on bone loss in experimental periodontitis and on osteoclastogenesis, activation and function.
What techniques and methods are used?
Used Techniques of the Project (1) Experimental periodontitis in rat and mice. (2) Histological and immunohistochemistry analysis. (3) Osteoclast culture systems. (4) Cell cytotoxity assay. (5) Osteoclast functional assays including tartrate-resistant acid phosphatase (TRAP) activity and staining, pits formation, and gelatin zymography assay. (6) Reverse transcription polymerase chain reaction (RTPCR), western blot, immunoprecipitation
What is the role of the student?
- The tasks will be done under supervision
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
The tasks that the student has to perform on his/her own including cell culture, cell cytotoxity assay, osteoclast functional assays including TRAP activity and staining, pits formation, and gelatin zymography assay, RTPCR, western blot, immunoprecipitation. The work that has to be done under supervision includes experimental design and discussion, animal experiments, histological and immunohistochemistry analysis, data acquisition and analysis
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
A scientific report is demanded after the exchange
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
- Mau LP; Cheng WC; Chen JK; Shieh YS; Cochran DL; Huang RY. Curcumin ameliorates alveolar bone destruction of experimental periodontitis by modulating osteoclast differentiation; activation and function. Journal of Functional Foods 2016: 22: 243?256
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