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Evaluation of the anti genotoxic and protective effect of vitamin E and beta-carotene in garbage recyclers exposed to pollution in the Cateura rubbish dump, for the essay of the comet and the test of micronucleus
Paraguay (IFMSA Paraguay) - Universidad Catolica de Asuncion
Genetic Toxicology Laboratory, Faculty of Health Sciences - Facultad de Ciencias de la Salud, Universidad Catolica de Asuncion.
Deidamia Franco de Diana
Deidamia Franco, Jaime Segovia, Celeste Vega, Diana Castiglioni
Portuguese, Spanish, Italian
Type of Research Project
- Basic science
What is the background of the project?
The laboratory works with a community of garbage recyclers, and in a preliminary investigation it was demonstrated that the people who work in the Cateura rubbish dump present DNA damage. The second phase of the research is to determine if the administration of vitamin E and beta - carotene has an antigenotoxic and protective effect in the same population.
What is the aim of the project?
to acquire knowledge and skills in the development of bioassays proposed for the detection of DNA damage
What techniques and methods are used?
In vivo bioassays: micronucleus assay and nuclear abnormalities in mucous cells, comet assay "single cell electrophoresis essay" in peripheral blood
What is the role of the student?
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
the student will participated in research projects developed within a scientific initiation program. It is expected that the student can join the group of laboratory members, acquiring knowledge ans contributing with their own knowledge
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student should have already studied subjects such as cell Biology and Genetics. It is expected that the student can join the group of laboratory members, acquiring knowledge and contributing with their own knowledge to the research. Biosecurity knowledge to work at the Laboratory. The student must bring his/her lab coat. The student must bring his/her logbook.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
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