Projects
Name
Biochemistry of intracellular transport: Biogenesis and autophagic degradation of Peroxisomes
University
Germany (bvmd) - Ruhr-Universitaet, Bochum
Domain
Biochemistry
Departement
AG Biochemie Intrazellulaerer Transportprozesse. Medizinische Fakultaet der Ruhr Universitaet Bochum. Universitaetsstr. 150, 44780 Bochum, MA / 03 / 141
Head
Jun.-Prof. Dr. Harald W. Platta
Tutor
Dr. Platta and PhD students
Languages
English, German
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
yes yes yes No No yes No yes yes yes yes No
Type of Research Project
- Basic science
What is the background of the project?
Peroxisomes contain at least 50 different enzymes, which are involved in a variety of biochemical pathways in different types of cells. The project deals with the transport of newly synthesized proteins from the cytosol over the peroxisomal membrane into the peroxisomal lumen for peroxisomal biogenesis (Project Part 1) and with the transport of the entire peroxisome to the yeast vacuole for autophagic degradation of aberrant organelles (Project Part 2)
What is the aim of the project?
The general aim of the project is to understand the protein transport processes required for the formation as well as the degradation of peroxisomes. A special focus lies on the posttranslational modifications of key factors via ubiquitination and phosphorylation.
What techniques and methods are used?
Methods in biochemistry (protein purification, protein-protein interaction studies), molecular biology (cloning and mutation of genes) and cell biology (yeast cell culture, fluorescence microscopy) will be used.
What is the role of the student?
What are the tasks expected to be accomplished by the student?
-
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
1
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student should already have a general theoretical background in biochemistry, biology or chemistry. After the exchange, the student can write a short report or an extended discussion of the results within the personal lab journal.
Are there any legal limitations in the student’s involvement
No
Hours
7
Type of students accepted
This project accepts:
- Medical students
- Pre-Medical students from the American-British system
Articles
- Suppressor Function of the Class III Phosphatidylinositol 3Kinase Complex by Ubiquitin and SUMO. Reidick; C.; El Magraoui; F.; Meyer; H.E.; Stenmark; H. and Platta; H.W. Cancers (Basel) 7; 1-29 (2015)
- The cycling peroxisomal targeting signal type 1 receptor Pex5p: reaching the circle`s end with ubiquitin. Platta; H.W. Receptor. Clin. Invest. 1; 55 til 68 (2014)