Projects
Name
The Teratogenic Effect of Anti-epileptic drug on the Developing Chick Embryo
University
Thailand (IFMSA-Thailand) - Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok
Domain
Pharmacology
Departement
Anatomy
Head
Assoc. Prof. Dr. Jantima Roongruangchan
Tutor
Assoc. Prof. Dr. Jantima Roongruangchan
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No Yes Yes No No No No No
Type of Research Project
- Basic science
What is the background of the project?
Anti-epileptic drugs are known for the risk of teratogenicity. Topiramate is a new kind of such drug, which no research has confirmed the incidence of producing congenital abnormalities. This study is conducted to study the teratogenic effects of Topiramate (TPM) by using chick embryos as animal model and the results can be compared to the human embryo of the same stage. The study is conducted on fertilized Leghorn hen eggs that were injected in ovo with three concentrations of TPM: 5mg, 10mg, and 20mg, in normal saline solution 0.1 mL, into the yolk sac at 23 hours of incubation and repeated injections at 72 hours at a volume of 0.05mL. The chick embryos on days 3, 6, and 11 of incubation are sacrificed and all living tissues are processed for total mount and serial section.
What is the aim of the project?
This study is conducted to study the teratogenic effects of Topiramate (TPM) by using chick embryos as animal model and the results can be compared to the human embryo of the same stage.
What techniques and methods are used?
Fertilized Leghorn hen eggs are injected in ovo with three concentrations of TPM, 5mg, 10mg, and 20mg, in NSS (normal saline solution) 0.1mL into the yolk sac at 23 hours of incubation and repeated injections at 72 hours at a volume of 0.05mL. The chick embryos on days 3, 6, and 11 of incubation are sacrificed and all living tissues are processed for total mount and serial section.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
Students should be able to set up lab of chick embryo experiment in the future. Students should also know how to mount a permanent slide.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
1
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic laboratory skills and biochemistry knowledge
Are there any legal limitations in the student’s involvement
No
Hours
7
Type of students accepted
This project accepts:
- Medical students
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