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Molecular diagnostic of Trichomona vaginalis in Pregnant and non pregnant women from Pedregal, District , Panam
Universidad de Panama
Dra. Maria Muñoz
Prof. Dayra Alvarez
English or Spanish
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Trichomona vaginalis is a parasitic protozoon that infects the human genitourinary tract, with vaginal and urethral secretions causing a sexually transmitted infection (STI). This infection generally shows non-specific signs and symptoms. Vaginal trichomoniasis has been linked to preterm birth and acquisition of HIV. Direct observation of this flagellate from vaginal smear shows a limited sensivity, however, it remains, to be the most widely diagnostic test for this infection. Culture of the parasite from vaginal smear is currently the
What is the aim of the project?
To determine the prevalence of Trichomona vaginalis in vaginal smears from Pregnant and Non Pregnant women from Pedregal District, Panama 2016.
What techniques and methods are used?
Gram Stain from vaginal samples. Giemsa Stain from vaginal samples. DNA Extraction from vaginal swabs (Wizard ® Genomic DNA Purification Kit). PCR with specific Primers for diagnostic of Trichomona vaginalis. Gel Electrophoresis to visualizate the amplificated product.
What is the role of the student?
What are the tasks expected to be accomplished by the student?
The student will work in a molecular biology laboratory. Will learn about the proper use and applications of molecular biology tools. Will work with DNA extraction from vaginal swab samples and PCR technique. The student, will review some new literature about the theme that will be presented to the Parasitology Professor from the department. The student will participate in some classes and will share with our medical students in a designated laboratory.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student must be coursed Medical Parasitology and Molecular Biology.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
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