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Screening of Pseudomonas aeruginosa strains derived from patients suffering from Cystic Fibrosis for protease activities
Germany (bvmd) - Ruprecht-Karls-Universitaet, Heidelberg
Dept. of Infectious Diseases, Medical Microbiology and Hygiene, University Hospital Heidelberg, Ruprecht-Karls University Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
Head: Prof. Dr. K. Heeg, Principal Investigator: Prof. Dr. A. Dalpke
Dr. Michael Weitnauer, cand. med. Julia Kantorek
Type of Research Project
- Basic science
What is the background of the project?
The project analyses potential immune escape mechanisms of Pseudomonas aeruginosa that can cause chronic infections in patients suffering from cystic fibrosis (CF). It has been shown that Pseudomonas aeruginosa secretes proteases that destroy soluble mediators (cytokines) of the immune system thereby abolishing an effective immune response by the host. We want to screen different protease activities in Pseudomonas in order to get to more insight in the different escape mechanism.
What is the aim of the project?
The aim is to screen a collection of Pseudomonas aeruginosa strains isolated from CF patients for protease activities.
What techniques and methods are used?
In the project we use cultivation of bacteria, plate susceptibility assays, gelelectrophoresis and western blotting to analyse the protease activities.
What is the role of the student?
What are the tasks expected to be accomplished by the student?
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge in microbiology and immunology recommended; no specific skills needed; it is expected that experiments are correctly noted in a lab book (Good Laboratory Practice) For further information please read: LaFayette SL, et al.. 2015. Cystic fibrosis-adapted quorum sensing mutants cause hyperinflammatory responses. Sci Adv 1. 2. Ruffin M, et al.. 2016. Quorum-sensing inhibition abrogates the deleterious impact of Pseudomonas aeruginosa on airway epithelial repair. FASEB J. in press 3. Weitnauer M, et al. 2016. Control of local immunity by airway epithelial cells. Mucosal Immunol 9:287-298
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
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