Projects
Name
Screening for host signaling pathways regulated by Toxoplasma gondii infection in human retinal pigment epithelium cell
University
Korea (KMSA) - Chungnam university, Daejeon
Domain
Parasitology
Departement
Department of Infection Biology. School of Medicine, Chungnam National University, 266, Munhwa-ro, Jung-gu, Daejeon, 35015, Korea
Head
Guang-Ho CHA, Ph.D.
Tutor
Guang-Ho CHA, Ph.D.
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
Yes Yes No No No No Yes Yes No No No No
Type of Research Project
- Basic science
What is the background of the project?
Background information : Toxoplasma gondii is a parasitic cell which can infiltrate into the host cell and proliferate inside. Usually, they are removed by immune system of human but in case of immune deficiency they can cause disease called Toxoplasmosis. In our lab, we study retinal toxoplasmosis by usinig retinal pigment epithelium cell line as model for infection and study how Toxoplsma gondii can leave in high reactive oxygen species environment of eye. - Aim : In this project, we would like to study what kind of intracellular signaling responses will be activated by infection of parasitic protozoan, Toxoplasma gondii, and try to find out the parasite unique cellular event , compare to bacterial infection. We are especially interested in intracellular signaling pathways which are well-defined in cancer or metabolism disease but not yet in immune related events. - Used Techniques and methods : Culturing the parasite, Toxoplasma gondii and host cell line is absolutely necessary to perform the experiments. And also, basic Western blot analysis or immunohistochemistry will be performed with certain phosphor-specific antibody to detect which signal pathways are activated or not.
What is the aim of the project?
In this project, we would like to study what kind of intracellular signaling responses will be activated by infection of parasitic protozoan, Toxoplasma gondii, and try to find out the parasite unique cellular event, compare to bacterial infection. We are especially interested in intracellular signaling pathways which are well-defined in cancer or metabolism disease but not yet in immune related events.
What techniques and methods are used?
Culturing the parasite, Toxoplasma gondii and host cell line is absolutely necessary to perform the experiments. And also, basic Western blot analysis for immunohistochemistry will be performed with certain phosphor-specific antibody to detect which signal pathways are activated or not.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Basic lab skill in molecular biology or biochemistry and the knowledge about the immunology would be helpful.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No legal limitation, scientific report required (Preliminary reading and introductory lectures will be provided before the lab work)
Are there any legal limitations in the student’s involvement
No
Hours
8
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
- Students in biomedical fields
Articles
- Zhou W; Quan JH; Lee YH; Shin DW; Cha GH. ( 2013) Toxoplasma gondii Proliferation Require Down-Regulation of Host Nox4 Expression via Activation of PI3 Kinase/Akt Signaling Pathway. PLoS One. 8(6):e66306