Projects
Name
Role of reactive oxygen species in autoimmune diseases
University
Korea (KMSA) - Chungnam university, Daejeon
Domain
Biochemistry
Departement
Department of Medical Education. School of Medicine, Chungnam National University, 266, Munhwa-ro, Jung-gu, Daejeon, 35015, Korea
Head
Sung-Soo JUNG M.D.
Tutor
Jae-yul KWON Ph.D.
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
Yes Yes No No No No Yes Yes No No No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
- Description : Elevated level of reactive oxygen species have been observed in the immune cells of several autoimmune diseases. In this project, we assess the level of reactive oxygen species in the immune cells from rheumatoid arthritis patients through the multiparametric flow cytometry and elucidate the role of reactive oxygen species in the pathogenesis of rheumatoid arthritis. - Background information of the project : Sustained and higher production of reactive oxygen species and weakened antioxidant defense systems have been observed in the several situations of chronic inflammation. This oxidative stress is thought to have an important role in the pathogenesis of many autoimmune diseases. - Aim : (1) To assess the correlation between higher production of reactive oxygen species and rheumatoid arthritis. (2) To identify the role of Reactive oxygen species in the pathogenesis of rheumatoid arthritis. - Used techniques and methods : We apply flow cytometry to the study of cells. Different dyes will be used to measure the level of reactive oxygen species in the cells from patients and healthy donors. To identify the specific cell types multiparametric flow cytometry will be used for immunophenotyping.
What is the aim of the project?
1) the assess the correlation between higher production of reactive oxygen species and rheumatoid arthritis. 2) To identify the role of Reactive oxygen species in the pathogenesis of rheumatoid arthritis.
What techniques and methods are used?
We apply flow cytometry to the study of cells. Different dyes will be used to measure the level of reactive oxygen species in the cells from patients and healthy donors. To identify the specific cell types multiparametric flow cytometry will be used for immunophenotyping.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
He/She will perform the experiments to measure the level of reactive oxygen species in the samples and analyze data, eventually to write the scientific report and present the result in the lab meeting.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary readings and introductory lectures will be provided before the lab work
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No legal limitation Scientific report required (Preliminary readings and introductory lectures will be provided before the lab work)
Are there any legal limitations in the student’s involvement
No
Hours
8
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
- Students in biomedical fields
Articles
- Gelderman KA; Hultqvist M; Olsson LM; Bauer K; Pizzolla A; Olofsson P; Holmdahl R. Rheumatoid arthritis: the role of reactive oxygen species in disease development and therapeutic strategies. Antioxid Redox Signal. 2007; 9:1541-67.
- Julien Cachat; Christine Deffert; Stephanie Hugues; Karl-Heinz Krause. Phagocyte NADPH oxidase and specific immunity. Clinical Science; 2015. 128:635-648.
- Eruslanov E; Kusmartsev S. Identification of ROS using oxidized DCFDA and flow-cytometry. Methods Mol Biol. 2010. 594:57-72