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The role of MIF protein family in cardiovascular diseases and stroke
Germany (bvmd), Ludwig-Maximilians-Universitaet, Munich
Department of Vascular Biology, Institute for Stroke and Dementia Research (ISD) Ludwig-Maximilians-University of Munich (LMU), Feodor-Lynen-Straße 17, D-81377 Munich, Germany
Prof. Dr. Jürgen Bernhagen
Dr. rer. nat. Omar El Bounkari, email@example.com
Type of Research Project
- Basic science
What is the background of the project?
The internship will be in the context of vascular biology and will mainly be a part of a current running project dealing with the characterization of the role of chemokine-like function chemokine MIF protein family in the dynamic migratory behaviour, recruitment and cell motility of immune cells in cardiovascular diseases and stroke. MIF (macrophage migration inhibitory factor) is well admitted as a key mediator of inflammatory and vascular diseases which is mainly associated with recruitment of immune cells including monocyte and lymphocytes to the site of inflammation. Recently, increasing studies suggested the involvement of MIF in stroke and neurodegenerative iseases such as Alzheimer. However, the impact of MIF and MIF-2 on the dynamic migratory behaviour, recruitment and cell motility of immune cells in stroke remains unclear. During his traineeship the candidate will have the opportunity to perform different in vitro experiments that include a variety of biochemical, immunological and cell biology methods.
What is the aim of the project?
The aim of the project will be to further characterize the cardioprotective properties of MIF within myocardial ischemia/reperfusion and to identify possible mechanisms including such as medications that might inhibit the MIF secretion in patients undergoing cardiac surgery. We will test the influence of various medications on MIF secretion in cardiomyocytes.
What techniques and methods are used?
We use experimental models: isolation of cardiomyocytes (out of mice), cell culture, preconditioning and clinical studies. We measure cytokine levels by the means of selfestablished ELISA (ezyme-linked Immunosorbent Assay), quantification PCR (polymersase chain reaction), Western-Blot, microscopy and FACS (fluorescence activated cell sorting) Analysis.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
- Knowledge about the literature and the methods related to the project performed. - Knowledge about experiment and concept design including analytical evaluation of the result obtained. - Presentation of obtained results as a progress report
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary reading, Attendance to several Seminars (weekly) and scientific talks (weekly) and intern Mini Journal Club (Monthly) etc..
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
We expect high motivation and we will send the student literature that should be read in advance. English is mandatory. Experience in the lab and life sciences are preferable.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Pre-Medical students from the American-British system
- Please see in Pubmed: Stoppe; Bernhagen 2012
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