Projects
Name
Versican gene silencing implication in trophoblast cell functions
University
Federal University of Alagoas (UFAL)
Domain
Genetics
Departement
Department of Cellular and Molecular Biology, Institute of Biological Sciences and Health, Federal University of Alagoas
Head
Iracilda Maria Moura Lima, PhD
Tutor
Alexandre Urban Borbely, PhD
Languages
English, Spanish, Portuguese
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
yes yes yes yes yes yes yes yes yes yes yes yes
Type of Research Project
- Basic science
What is the background of the project?
Increased or decreased trophoblast invasiveness during human pregnancy implicate in clinically relevant pathologies that might lead to maternal death. Amid these pathologies, it is possible to highlight preeclampsia, abnormally invasive placenta (AIP), invasive mole and choriocarcinoma, all presenting trophoblast invasiveness-related problems. Accumulating evidence suggests trophoblast cells have biological properties similar to neoplastic cells, although their migration and invasion are regulated in a temporally and spatially controlled manner. Despite all gestational pathologies have different causes which are still debated or unknown, a plethora of molecules are acknowledged to negatively or positively affect trophoblast behavior, among them, versican. Recent unpublished data from our group show different versican isoforms expression in these pathologies, mainly in AIP, which represents a high amount of maternal hemorrhage morbidity and mortality worldwide.
What is the aim of the project?
Herein it is aimed to analyze versican functions in different trophoblast cells, using first trimester and choriocarcinoma derived cell lines, as well as primary trophoblast cultures from preeclampsia and term placentas to verify versican gene silencing implications in trophoblast differentiation, proliferation, death, migration, invasion and cytokines production.
What techniques and methods are used?
For that, will be used primary and cell line cultures, siRNA gene silencing, PCR assay, histological stainings, ELISA assay, immunofluorescence, flow cytometry and in vitro assays to access trophoblast proliferation, death, migration, invasion and differentiation.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
For that, will be used primary and cell line cultures, siRNA gene silencing, PCR assay, histological stainings, ELISA assay, immunofluorescence, flow cytometry and in vitro assays to access trophoblast proliferation, death, migration, invasion and differentiation.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
1
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Proactivity, commitment and responsibility. In addition, it is required basic knowledge in histology, immunology and cell biology. A scientific report is not demanded, but is highly recommended. No legal limitations, the project already was approved by the ethical committee in human research.
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
Articles
- BORBELY; A.U. et al. The term basal plate of the human placenta as a source of functional extravillous trophoblast cells. Reprod Biol Endocrinol. Jan 2014. 28;12:7.
- BORBELY; A.U. et al. Decorin and biglycan immunolocalization in non-villous structures of healthy and pathological human placentas. Histopathology. Apr 2014. 64(5):616-25.
- SANDRI; S. et al. Serum amyloid A in the placenta and its role in trophoblast invasion. PLoS One. Mar 2014. 10;9(3):e90881.