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Immunohistochemical and image analyses of neuronal and glial cells of the spinal cord of mice carrying a transgene for mutant superoxide dismutase 1 related to amyotrophic lateral sclerosis (ALS) and nontransgenic littermates
Tokyo Women's Medical University
Department of Pathology, Tokyo Women
Noriyuki Shibata, M.D., Professor and Chairman, Department of Pathology
Yoichiro Kato, Ph.D., Assistant Professor, Department of Pathology
Type of Research Project
- Basic science
What is the background of the project?
Description: Yoichiro Kato, Ph.D., Assistant Professor, Department of Pathology Aim: The aim of this project is to compare quantitatively the number of neurons, astrocytes and microglia in the spinal cord ventral horns between young and old ALS and control mice, using morphological and quantitative approaches. Techniques: Immunohistochemical staining, recording microphotographs, and quantitative verification using an image software will be used.
What is the aim of the project?
The aim of this project is to compare quantitatively the number of neurons, astrocytes and microglia in the spinal cord ventral horns between young and old ALS and control mice, using morphological and quantitative approaches.
What techniques and methods are used?
Immunohistochemical staining, recording microphotographs, and quantitative verification using an image software will be used.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
It is expected that the student will be able to achieve immunohistochemical staining and image analysis himself/herself, and that he/she will consider, verify and assess the data obtained, leading to a conclusion and the next question.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
We are ready to lecture a principle and methods of immunohistochemical staining and a technique of handling the software.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No special skill is needed before the project except for a basic knowledge of antigen-antibody binding reaction and peroxidase reaction.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Increasing communication skills with persons from other countries
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