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[Meridad] Urban house environment as a source of vector-borne diseases in Merida, Mexico
Universidad Autonoma de Yucatan
Laboratorio de Enfermedades Infecciosas y Parasitarias I. Facultad de Medicina.
Dr. Gaspar Fernando Peniche Lara
Theory: Dr. Gaspar Fernando Peniche Lara; Research: Dr. Gaspar Fernando Peniche Lara; Fieldwork: Dr. Gaspar Fernando Peniche Lara
English and Spanish
Type of Research Project
- Global Action Project (GAP)
What is the background of the project?
Rickettsioses, ehrlichioses and babsesises are common febrile illness in Yucatan State in rural areas. Urban areas in Yucatan State are neglected areas of vector-borne diseases. In this project we will identify the presence of Rickettsia, Babesia and Ehrlichial species in ticks and fleas samples from rural areas and reservoir and serum in human samples.
What is the aim of the project?
To identify and characterize rickettsiosis, babesiosis and ehrlichiosis in rodents and domestic animals and their ectoparasites in urban households from Merida City, Mexico. To estimate prevalence of rickettsiosis, babesiosis and ehrlichiosis in an urban community of Merida City, Mexico.
What techniques and methods are used?
Epidemiological research that includes (1) sample collection trip to urban and rural areas in Yucatan States (2) molecular diagnostic of rickettsial diseases by real time polymerase chain reaction, conventional polymerase chain reaction, electrophoresis, restriction analysis polymorphism (3) DNA cloning and sequencing, (4) Bioinformatic analysis of sequence obtained. In all the procedures and experiments, the student first will observe and be trained in all techniques and after completed the training, the student will be involved in the project in all the activities proposed working directly with the samples..
What is the role of the student?
What are the tasks expected to be accomplished by the student?
The student will visit a rural community (Mayapan community, a small town in Yucatan State, Mexico) and urban areas from Merida, Capital from Yucatan State. In the field work he will inspect domestic animals like dogs, cats, cows of horses to obtain ectoparasites (tick of fleas). He will collect epidemiological data from house visited like number of family members, type fo floor or walls, location of the kitchen, and concts that will be associated to ectoparasite presence in house to compare the data obtained from rural and urban houses. - The student actively participate in the project collaborating in the sample collection - DNA extraction and purification - molecular diagnostic by conventional and real-time polymerase chain reaction for rickettsial diseases and bioinformatic analysis
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Pre-Medical students from the American-British system
- Chikeka I1; Dumler JS2. Neglected bacterial zoonoses. Clin Microbiol Infect. 2015 May;21(5):404-415. doi: 10.1016/j.cmi.2015.04.022. Epub 2015 May 8
- Zavala-Castro J; Zavala-Velazquez human infection with Rickettsia felis associated with hepatitis in Yucatan; Mexico. 2009. International JouJ; Walker D; Perez-Osorio J; Peniche-Lara G. Severe rnal of Medical Microbiology. 299(7). Noviembre de 2009; pp 529-533
- Zavala-Castro J; Zavala-Velazquez J; Sulu-Uicab E; Peniche-Lara G. Human cases of Rickettsialpox in the Southeastern of Mexico. 2009. Emerging Infectious Diseases. 15(10) October2009; pp 1665 ? 1667.
- Peniche-Lara G; Zavala-Velazquez J; Dzul-Rosado K; Walker DH; Zavala-Castro J. Simple method to differentiate among Rickettsia species. 2013. J Mol Microbiol Biotechnol 2013;23(3):203-8. doi: 10.1159/000348298. Epub 2013 Apr 13.
- Dzul-Rosado K; Gonzalez-Martinez P; Peniche-Lara G; Zavala-Velazquez J; Zavala-Castro J. Murine typhus in humans ; Yucatan Mexico. Emerg Infect Dis. 2013 Jun;19(6):1021-2. doi: 10.3201/eid1906.121400.
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