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Evaluation of immunoregulatory and anti-cancer potential of nanoparticles in reference to healthy peripheral blood cells and different cancer cell lines respectively.
Poland (IFMSA-Poland) - Medical University of Bialystok, Bialystok
Department of Regenerative Medicine and Immune Regulation, Jerzego Waszyngtona 13, 15-269 Bialystok
prof. Marcin Moniuszko
prof. Marcina Moniuszko i dr Kamila Grubczaka
Type of Research Project
- Basic science
What is the background of the project?
Our project will concentrate on assessment of nanoparticles properties in reference to cancer cells and healthy blood donors cells to establish their possible use in novel anti-cancer and immunomodulatory therapeutic approaches as carriers and factors amplifying activity of other anti-cancer drugs.
What is the aim of the project?
The aim of the project is to evaluate in in vitro conditions the properties of different kinds of nanoparticles, alone or in combination with compounds bearing potential anti-cancer activity, on the phenotype and function of various cancer cell lines, including inter alia lung, colon, breast cancer, and melanoma.
What techniques and methods are used?
? Isolation of peripheral blood cells. ? Maintenance of cancer cell lines. ? Conducting in vitro cell cultures. ? Flow cytometry. ? Confocal microscopy. ? Cell sorting using fluorescence-activated cell sorting. ? PCR technique. ? Immunoenzymatic technique - ELISA.
What is the role of the student?
What are the tasks expected to be accomplished by the student?
Participation and self-development.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Student must have at least basic knowledge of laboratory work and the principles of working with biological material. Experience with handling blood specimen and isolation of peripheral blood cells would be essential. We will demand a scientific report at the end of the exchange. We expect full commitment of the student to the described project and participation in every single stage of the research. High self- discipline and ambitious approach to the project-associated duties and expanding student knowledge.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
- Adan A1; Alizada G2; Kiraz Y1;2; Baran Y1;2; Nalbant A2. Flow cytometry: basic principles and applications. Crit Rev Biotechnol.2016 Jan 14:1-14.
- Wong ML1; Medrano JF. Real-time PCR for mRNA quantitation.Biotechniques. 2005 Jul;39(1):75-85.
- Gillet JP1; Varma S; Gottesman MM. The clinical relevance of cancer cell lines.J Natl Cancer Inst. 2013 Apr 3;105(7):452-8.
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