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Modulation of signaling pathways modulating intracellular energy balance in tumor therapy and neuro-immuno-endocrine disorders
Serbia (IFMSA-Serbia) - University of Belgrade, Belgrade
Laboratory of immunology, Institute of Microbiology and immunology, School of Medicine, Pasterova 2, 11000 Belgrade, Serbia
Prof. Vladimir Trajkovi
Prof. Vladimir Trajkovic
Type of Research Project
- Basic science
What is the background of the project?
Autophagy is an essential metabolic pathway by which the intracellular unwanted materials are digested within lysosomes for cellular homeostasis. It provides energy and building blocks upon starvation or other stresses. Autophagy even contributes to cell death especially under apoptosis incompetent conditions. Dysfunction of autophagy involves the initiation and progression of multiple diseases and their treatments. Basal autophagy may serve as a tumor suppressive mechanism during tumorigenesis; nevertheless, excessive autophagy even works as a pro-survival pathway in already established cancers. Recently, mounting evidence highlighted its key roles in the genesis and therapy of various hematological malignancies. Our project is focused on intracellular energy balance in various system models (from cell lines to human studies) with accent on regulation of AMP-dependant protein kinase (AMPK) and authophagy.
What is the aim of the project?
The aim of the project is to explore posibilities of modulation of AMPK activity and autophagy by especially by drugs already used in humans, but typicaly not for cancer or neuro-immuno- endocrine disorders (e.g. antipsychotics)
What techniques and methods are used?
Cell culture, cell viability tests, Western blot, nucleic acid isolation, qPCR and flow cytometry will be used.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Student will be introduced to the basics of the life science research and obtain training in some of the state-of- the-art techniques (as mentioned above). The student is expected to be highly motivated for the collaborative laboratory work and ready to accept the chalenge of interdiscipllinary research. He/she will have the chance to grow cell culture, do cell viability tests, work on Western blot, nucleic acid isolation, qPCR and flow cytometry. Student is also expected to do the search of the literature, and discuss it with mentor.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, preliminary readings
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Vucicevic L; Misirkic-Marjanovic M; Paunovic V; Kravic-Stevovic T; Martinovic T; Ciric D; Maric N; Petricevic S; Harhaji-Trajkovic L; Bumbasirevic V; Trajkovic V. Autophagy inhibition uncovers the neurotoxic action of the antipsychotic drug olanzapine. Autophagy. 2014;10:2362-78.
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