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Immune checkpoints in the pathogenesis of post-transplant neoplastic disease
Universita degli Studi di Foggia
Department of Surgical and Medical Sciences Centro di Medicina Molecolare, Blocco B, II piano, Ospedali Riuniti di Foggia, Viale Luigi Pinto, 1 - 71100 Foggia
Prof. Maria Pia Foschino
Prof. Elena Ranieri, Dott.ssa Federica Rascio, Prof. Grandaliano
Type of Research Project
- Basic science
What is the background of the project?
Malignancies represents the third cause of mortality among kidney transplant recipients worldwide. Although the role of the immune system in tumor progression is widely confirmed, the mechanisms of tumor escape from the immune system remains unclear. Starting from a microarray-based approach, we will validate data by real-time polymerase chain reaction (qPCR), enzyme-linked immunosorbent assay (ELISA), and confocal microscopy in order to discover new immunological markers useful to identify patients at risk of developing PTM (Post-Translational Modifications).
What is the aim of the project?
The aim of the study is to evaluate, through the analysis of gene expression profiles in peripheral mononuclear cells (PBMC), alterations of immune responses in post-kidney transplant malignancies (PTM) patients compared to transplanted patients without malignancies (Ctrl-Tx), no-transplanted malignancies (NTM) patients and healthy subjects.
What techniques and methods are used?
The main molecular biology techniques that will be applied are: PBMC isolation - mRNA extraction - qPCR - ELISA - Immunofluorescence and confocal microscopy - western blotting
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Active participation to the activities of the lab (validation of microarray data). It is expected that the student understand the aim of the project
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Theoretical basis of molecular biology, cancer and immunology ? Murugaiyan G, Saha B. IL-27 in tumor immunity and immunotherapy. Trends Mol Med. 19:108-16, 2013. ? Vajdic CM, McDonald SP, McCredie MR, van Leeuwen MT, Stewart JH, Law M, Chapman JR, Webster AC, Kaldor JM, Grulich AE. Cancer incidence before and after kidney transplantation. JAMA 296: 2823?2831, 2006 ? Twine NC, Stover JA, Marshall B, Dukart G, Hidalgo M, Stadler W, Logan T, Dutcher J, Hudes G, Dorner AJ, Slonim DK, Trepicchio WL, Burczynski ME. Disease-associated Expression Profiles in Peripheral Blood Mononuclear Cells from Patients with Advanced Renal Cell Carcinoma. Cancer Research. 63: 6069-6075, 2003
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
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