Phylogenetic approach to study of highly variable viruses
Serbia (IFMSA-Serbia) - University of Belgrade, Belgrade
Virology laboratory, Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, street Dr Subotica starijeg N
Maja Stanojevic, Associate professor
Maja Stanojevic, Associate professor
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No Yes No No No No No Yes Yes Yes Yes No
Type of Research Project
- Basic science
What is the background of the project?
_ Molecular evolution involves structural changes in macromolecules induced in a process of selection and adaptation that are further manifested as resistance to antiviral drugs, immune escape mechanisms, viral tropism changes, diverse host-pathogen interactions etc. Study of molecular evolution provides an insight into the basis and extent of variability, allows the establishment of correlations between molecular phenomena and their phenotypic expression using diverse models of evolution within phylogenetics, phylodynamics, phylogeography.
What is the aim of the project?
The main objective of this study is to analyze aspects of molecular evolution of highly variable viruses, such as human immunodeficiency virus (HIV) and coinfecting viruses and hantaviruses, using methods of phylogenetic analysis (phylogenetics, phylodinamics, phylogeography), particularly in the context of co-infection and virus-host interaction
What techniques and methods are used?
Molecular methods for viral genome detection such as PCR, RT-PCR, DNA sequencing and bioinformatic methods fro phylogenetic and evolutionary analyses.
What is the role of the student?
What are the tasks expected to be accomplished by the student?
According to particular research question exchange students will be included in benchwork and in bioinformatic analyses. The student will acquire some experience in moleular methods (extraction of NA, PCR, gel ecetrophoresis); insight into bioinformatic tools for studying viral genomes
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Some introductionary guidance on the relevant subjects
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare an abstract
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge of molecular biology and virology is required.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
- Students in biomedical fields
- 1. Stanojevic M; Siljic M; Salemovic D; Pesic-Pavlovic I; Zerjav S; Nikolic V; Ranin J; Jevtovic D. Ten years survey of primary HIV-1 resistance in Serbia: the occurrence of multiclass resistance. AIDS Res Hum Retroviruses. 2014 Jul;30(7):634-41
- 2. Nikolic V; Stajkovic N; Stamenkovic G; Cekanac R; Marusic P; Siljic M; Gligic A; Stanojevic M. Evidence of recombination in Tula virus strains from Serbia. Infect Genet Evol. 2014 Jan;21:472-8