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Influence of Polyoxotungstates on Rat Synaptic Plasma Membrane ATPases Activity
Serbia (IFMSA-Serbia) - University of Belgrade, Belgrade
Institute of Medical Chemistry, Faculty of Medicine, Vi
Dr. Danijela Krsti
Dr. Danijela Krsti
Type of Research Project
- Basic science
What is the background of the project?
Polyoxometalates (POMs) are discrete, inorganic metal-oxo complexes that possess versatile biological activities, including antibacterial, anticancer, antidiabetic, and antiviral effects. The mechanism of action at the molecular level is largely unknown. It has been suggested that inhibition of several nucleotide-dependent enzymes (including ATPases) by POMs may contribute to their pharmacological properties such as anticancer activity. The objective of the project is the evaluation of the inhibitory potency of a series of polyoxotungstates towards two types of ATPases in order to find strong and selective ATPases inhibitors with a low level of side effects. Because of their roles in cell signaling, the chosen ATPases are suitable “targets” for evaluating the anti- cancer activity of the tested compounds.
What is the aim of the project?
In vitro evaluation of the inhibitory potency of a series of polyoxotungstates at two types of ATPases activity (Na+/K+-ATPase and ecto-ATPase) from rat synaptic plasma membrane
What techniques and methods are used?
Isolation of synaptic plasma membrane from rat brain, enzyme inhibition studies and spectrophotometric techniques
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Isolation of synaptic plasma membrane from rat brain, enzyme inhibition studies and spectrophotometric techniques. Practical lab skills (making solutions, performing enzyme reactions, spectrophotometry, differential centrifugation), keeping the laboratory notebook, statistical analysis – collecting and analyzing data, using program SPSS, writing a scientific report
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, preliminary readings
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Student should have listened or passed the exam in General Chemistry and Biochemistry
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- 1. https://www.researchgate.net/publication/40785950_Influence_of_decavanadate_on_rat_synaptic_plasma_membrane_ATPases_activity
- 2. https://www.researchgate.net/profile/Mirjana_Colovic/publication/51764822_Inhibition_of_rat_synaptic_membrane_NaK-ATPase_and_ecto-nucleoside_triphosphate_diphosphohydrolases_by_12-tungstosilicic_and_12-tungstophosphoric_acid/links/02e7e5278ad662e574000000.pdf
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