Projects
Name
Evaluation of immune mechanisms in experimental glomerulonephritis
University
Austria (AMSA) - University of Graz, Graz
Domain
Nephrology
Departement
Medical University of Graz, Clinical Division of Nephrology, Auenbruggerplatz 27, 8036 Graz, Austria
Head
Prof. Alexander Rosenkranz
Tutor
Ass.-Prof. PD Dr. Kathrin Eller
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes Yes No No No No
Type of Research Project
- Basic science
What is the background of the project?
Cells of the innate and adaptive immune system are critically involved in the pathogenesis of experimental glomerulonephritis (GN) also called nephrotoxic serum nephritis. We have shown that targeting different cell populations and immune mediators such as chemokines and cytokines offers new therapeutic options in GN. What is the aim of the project? Evaluation of the role of members of the IL-1beta family, such as IL-33 and IL-36. What techniques and methods will be used? Nephrotoxic serum nephritis will be induced in mice which will be treated with respective interleukins and their blocking antibodies. Mice will be followed for 14 days and evaluated for the phenotype of disease by evaluating albuminuria (by ELISA), histology (by PAS stain), immune cell infiltration (by immunohistochemistry) and cytokine profiles (by ELISA and real-time PCR)
What is the aim of the project?
Evaluation of the role of members of the IL-1beta family, such as IL-33 and IL-36.
What techniques and methods are used?
Nephrotoxic serum nephritis will be induced in mice which will be treated with respective interleukins and their blocking antibodies. Mice will be followed for 14 days and evaluated for the phenotype of disease by evaluating albuminuria (by ELISA), histology (by PAS stain), immune cell infiltration (by immunohistochemistry) and cytokine profiles (by ELISA and real-time PCR)
What is the role of the student?
What are the tasks expected to be accomplished by the student?
Learn how to induce glomerulonephritis in mice and perform evaluations (ELISA, histology, real time PCR, etc) under supervision of an experienced researcher of our lab group
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
1
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Motivation to perform basic research, ability to work in a team.
Are there any legal limitations in the student’s involvement
No
Hours
7
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
Articles
- Pubmed: publications Elller K et al. (nephrotoxic serum nephritis)