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[Sfax]The efficiency study of the ‘QuantiFERON-TB (QFT)’ new screening technique of mycobacterium tuberculosis infection
Tunisia (ASSOCIA-MED) - University of Sfax/ Faculty of Medecine of Sfax, Sfax
The immunology laboratory ,Habib Bourguiba university hospital of Sfax, Tunisia
Pr. Hatem Masmoudi
Dr. Sawsan Feki
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Tuberculosis is a contagious disease caused by mycobacterium tuberculosis infection typically transmitted by the evaporated droplets of ill patients. As this disease is still considered as a public health problem in Tunisia, this research project regarding the efficiency of the QFT screening technique is of a highly importance to our endemic country.
What is the aim of the project?
This research project aims to biologically and clinically study the efficiency of the ‘QuantiFERON-TB (QFT)’ assay as a new screening method of the mycobacterium tuberculosis infection in an endemic country that is Tunisia.
What techniques and methods are used?
The QFT assay is an indirect test for mycobacterium tuberculosis infection based on 3 steps: Special technique of taking blood samples in 3 tubes: negative control tube, positive control tube and TB antigen tube. Incubation 24h at 37°C Quantifying the amount of gamma interferon produced by each tube using ELISA format. The use of a special interpretation software is required.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The exchange student participating in this research project is expected to understand the principle, the technical steps, the indications as well as the clinical contribution of the ‘QuantiFERON-TB (QFT)’ assay according to its studied efficiency in screening tuberculosis in Tunisia. Also, to attend molecular biology techniques manipulation at first, and then participate actively to these manipulations with the supervision of tutors.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Bibliographic reference and round-table discussion about the topic of this research will be provided to the exchange student.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The exchange student participating in this project should be a clinical student that has already studied the subject of immunology during his/her medical education.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
- Articles in Pubmed about Mycobacterium tuberculosis infection
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