Projects
Name
Investigation of micro RNA gene expression profiles in patients with Crimean - Congo Hemorrhagic Fever
University
Turkey (TurkMSIC) - Cumhuriyet University, Sivas
Domain
Infectious Diseases
Departement
nternal Medicine - Infectious Diseases Department
Head
Prof. Dr. Mehmet BAKIR
Tutor
Prof. Dr. Mehmet BAKIR
Languages
English, Turkish
Duration
6 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes Yes Yes No No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Crimean Congo hemorrhagic fever (CCHF) is a disease caused by the Crimean Congo hemorrhagic fever vi rus (CCHFV) and characterized with sudden onset of high fever, s evere headache, dizziness, back and abdominal pain. The virus has single stranded RNA genome of negative polarity, belongs to the family Bunyaviridae, genus Nairovirus. MicroRNAs (miRNAs) are small noncoding RNAs responsible of post transcriptional regulation of gene expression through interaction with Messenger RNAs (mRNAs) . The role of miRNAs in both viral and host gene expression control in the infected cell is starting to be elucidated for some pathogenic viruses. These miRNA alterations can be used as useful biomarkers for diagnosis, prognosis and response to therapy during viral infection. The results obtained from this study will contribute to the explaination the role of miRNAs in the pathogenesis of Crimean ? Congo hemorrhagic fever vir us. Also, weaim that the results will be a s ource of data for the developement of antagomir - based therapy against CCHF infection and micro RNA usage in the future. What techniques and methods will be used? In our study, patients with CCHF will be investigated in miRNA expression profiles using microarray analysis at first. The research population will be composed of individual s with definitive diagnosis of CCHF infection. All CCHF patients w ill be classified into two groups in terms of disease severity (?severe?, ?non - severe?), according to severity scores reported by the tutor and his co - workers. In addition, the patient population will be discussed in terms of living and deceased patients. The micro - RNA expression levels among this three groups (?severe?, ?non - severe?, ?fatal?), will be compared within the case population .We also will investigate whether or not there is a correlation between mi RNA expression profiles and clinical data of patients t hus, the genes and pathways which are regulated by these micro RNAs will be determined with bioinformatics applications .
What is the aim of the project?
What techniques and methods are used?
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
1
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student needs to be clinical student. We expect her/him to be well aware of bed-side manners and laboratory security. There are no legal limitations in the student?s involvement in the project.
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
Articles
- http://www.nature.com/nrmicro/journal/v11/n3/full/nrmicro2971.html
- http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1003018
- http://bioinformatics.oxfordjournals.org/content/early/2013/08/07/bioinformatics.btt391.full