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The role of gammadelta T lymphocytes in immune responses in the lungs: from pathogen clearance to tissue damage control
Portugal (PorMSIC) - University of Lisbon, Lisbon
Imunologia Basica, Edificio Ega Moniz
Bruno Silva Santos
Type of Research Project
- Basic science
What is the background of the project?
The lungs harbour different populations of gammadelta T cells which exert their funtions mainly through the production of the cytokines Interleukin-17A (IL-17A) and Interferon gamma (IFN-gamma). During the course of different fungal and helminthic infections, IL-17-producing gammadelta T cells were shown to play an important role in pathogen clearance. However, little is known about their interactions with other pulmonary resident cells, such as the type-2 innate lymphoid cells (ILC2), responsible for the maintenance of lung homeostasis through generation of acute type-2 responses during infection and allergy. Additionally, preliminary data from our lab suggest that also IFN-gamma-producing gammadelta T cells are critical for the maintenance of tissue integrity during type-2 responses.
What is the aim of the project?
To dissect the crosstalk between IL-17-producing gammadelta T cells and ILC2 during immune responses to pathogens in the lungs; and to investigate the role of IFN- gamma-producing gammadelta T cells during tissue damage control.
What techniques and methods are used?
Mouse models for helminth infections and allergic responses; flow cytometry for lymphocyte populations; in vitro stimulation of lymphocytes.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
We’re seeking a highly motivated student to participate on the day-to-day activities of our lab, such as working with mouse models for helminth infections and allergic responses, flow cytometry for lymphocyte populations and in vitro stimulation of lymphocytes. Also, the student will have the opportunity to participate on the data analysis and scientific discussions regarding the project.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The students will have the opportunity to participate in the lab meetings and to attend the lectures taking place at the institute during the course of the internship.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic immunology knowledge is desirable, but not mandatory. No reports will be demanded afterwards, just an oral presentation describing the path in the lab and what the student has learned.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Ribot. J. C. et al. Cutting edge: adaptive versus innate receptor signals selectively control the pool sizes of murine IFN-gamma- or IL-17-producing gammadelta T cells upon infection. J Immunol 185. 6421-6425
- Ribot. J. C. et al. CD27 is a thymic determinant of the balance between interferongamma- and interleukin 17-producing gammadelta T cell subsets. Nature immunology 10. 427-436. doi:10.1038/ni.1717 (2009).
- Born W. K. et al. Role of gammadelta T cells in protecting normal airway function. Respiratory research 1 151-158 doi:10.1186/rr26 (2000).
- Dejima T. et al. Protective role of naturally occurring interleukin-17A-producing gammadelta T cells in the lung at the early stage of systemic candidiasis in mice. Infection and immunity 79 4503-4510 doi:10.1128/IAI.05799-11 (2011).
- Sutherland T. E. et al. Chitinase-like proteins promote IL-17-mediated neutrophilia in a tradeoff between nematode killing and host damage. Nature immunology 15 1116- 1125 doi 10 1038/ni 3023 (2014).
- Turner J. E. et al. IL-9-mediated survival of type 2 innate lymphoid cells promotes damage control in helminth-induced lung inflammation. The Journal of experimental medicine 210 2951-2965 doi:10.1084/jem.20130071 (2013).
- J. E. et al. IL-9-mediated survival of type 2 innate lymphoid cells promotes damage control in helminth-induced lung inflammation. The Journal of experimental medicine 210 2951-2965 doi:10.1084/jem.20130071 (2013).
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