[Aguascalientes] The effect of prebiotic or probiotic administration on experimental models of allergy.
Universidad Autonoma de Aguascalientes
Department of Microbiology
Dra. Eva Maria Salinas Miralles, PhD
Dra. Eva Maria Salinas Miralles, PhD
English and Spanish
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
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Type of Research Project
- Basic science
What is the background of the project?
The allergy prevalence of the whole population by country ranges from 10 to 40 %. Actual treatments to different allergy pathologies (as asthma, rhinitis, anaphylaxis, atopic dermatitis) are expensive, most of them have collateral effects and although control symptoms, mostly do not modify the illness course. There are intrinsic factors relating to the host (genes) and extrinsic factors relating to the environmental (allergen, diet, contamination) that may influence the development of allergy. Current hypothesis propose that microbiome is playing a crucial role in the protection to allergy, and gastrointestinal microbiome is the central microbial body habitat that influences allergy development at other body sites. Probiotic and prebiotic administration is a new therapeutic strategy to induce tolerance in allergy.
What is the aim of the project?
The aim of the project is to: -Analyze immunological and patho-physiological parameters related to allergic asthma and atopic dermatitis in rats with or without prebiotic or probiotic administration. -Determine changes in gut microbiota of normal and allergic rats, with or without prebiotic administration. -Analyze epigenetic modifications generated by prebiotic administration in allergic animals. -Determine the antioxidant properties of some prebiotics, using tumor line cells.
What techniques and methods are used?
Techniques and Methods used: Animals immunization and sensitization; cellular cultures; SDS- polyacrylamide electrophoresis; Immunoblot; immunohistochemistry; DNA/RNA purification and quantification; northern and southern blot; real time polymerase chain reaction PCR; ELISA assays; score of experimental allergic signs.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
- The student will be integrated to the laboratory work. First - the student will observe how to carry out different techniques and he/she will acquire skills to work in a basic research laboratory. When the student is ready - he/she will join to one of the projects in the lab to develop and practice the techniques in the context of the allergy study.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
A basic theoretical level in immunology.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Beguin P and Nadeau K C (2014) Epigenetic regulation of asthma and allergic disease. Allergic asthma & Clinical Immunology 10: 27.
- Jimenez M and Chavez N. Salinas E. (2012). Pretreatment with glycomacropeptide reduces allergen sensitization alleviates immediate cutaneous hypersensitivity and protects from anaphylaxis. Clinical and Experimental Immunology 170 (1): 18-27
- Legatzki A. Rosler B. Von Mutius E. (2014). Microbiome diversity and asthma and allergy risk. Curr Allergy Asthma Rep 14: 466.