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Melanoma stem cells biology
Poland (IFMSA-Poland) - Medical University of Gdansk, Gdansk
Department of Embryology, Medical University of Gdansk, Debinki 1 St, 80-210 Gdansk, Poland
Miroslawa Cichorek Ph.D
Miroslawa Cichorek Ph.D
Type of Research Project
- Basic science
What is the background of the project?
Tumor and non tumor origin stem cells biology is of special interest in a tumor biology as in a regenerative medicine. Tumor progression is not only the result of uncontrolled tumor cell proliferation but also a decreased ability to undergo programmed cell death especially tumor stem cells. This problem seems to be very important especially in chemotherapy-resistant tumors. Melanoma is a tumor of this kind and its chemoresistance is a serious clinical problem. As a model we use two hamster s transplantable melanoma lines of common origin but differing in many biological features. The first - melanotic melanoma line (Ma) has lower growth rate and immunogenicity, the second - amelanotic one (Ab), which appeared from the melanoma line during spontaneous alteration, is characterized by faster growth rate, higher tumorigenicity and shorter animal survival time. Such two forms of melanoma cells are presented also in human melanoma and appearance of amelanotic cells accompanies the progression of pigmented melanoma
What is the aim of the project?
This year project will be based on the detection of melanoma stem cells in melanotic and amelanotic form of hamster s melanomas and comparing theirs features with adipose tissue derived stem cells.
What techniques and methods are used?
Flow cytometry phenotypical characteristic of stem cells, immunodetecion based identification of these cells, western blott, tests for cells viability (tetrazolium dye XTT) and apoptosis (annexin V, fluorochrome-labeled inhibitor of caspases).
What is the role of the student?
What are the tasks expected to be accomplished by the student?
Participation and self-development.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Student is only expected to be interested in the lab work on the field of cells biology
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Pre-Medical students from the American-British system
- Cichorek M.; Kozlowska K.; Bryl E. Mitochondrial transmembrane potential in spontaneous and camptothecin- induced apoptosis of melanotic and amelanotic melanoma cells. Neoplasma 2007. 54 (1): 29-36.
- Cichorek M.; Kozlowska K.; Bryl E. The activity of caspases in spontaneous and camptothecin-induced death of melanotic and amelanotic melanoma cells. Cancer BiolTher 2007. 6 (3) 346-53
- Cichorek M. Camptothecin-induced death of amelanotic and melanotic melanoma cells in different phases of cell cycle. Neoplasma 2011.58. 3:227-234. IF 1.192
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