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Biophotonic analysis of persistent Staphylococcus aureus strains involved in chronic and difficult-to-treat infections
Germany (bvmd) - Friedrich-Schiller-Universitaet, Jena
Research Group and Core Unit Biophotonics, Center for Sepsis Control and Care (CSCC) Jena University Hospital Erlanger Allee 101, D-07747 Jena, Germany
Dr. Michael Bauer Head of research group: PD Dr. Ute Neugebauer
Dr. Christina Grosse
Type of Research Project
- Basic science
What is the background of the project?
Staphylococcus aureus infections often become chronic which is supposed to be associated with intracellular host cell infection and formation of small colony variants. This allows the bacteria to persist for long time in a quiescent state. Not much is known about the mechanisms of this persistence. Biophotonic techniques, such as fluorescence imaging and Raman spectroscopy are powerful tools to study the pathogenesis mechanisms. In particular Raman spectroscopy as a label free and non destructive spectroscopic method has several advantages to measure samples in their unprocessed state and obtain label free images as well as to discriminate unknown samples for diagnostic purposes
What is the aim of the project?
The project aims to use Raman spectroscopy and fluorescence imaging to shed light on the pathogenesis mechanisms of persistent S. aureus infections.This will involve biophotonic approaches to identify biomarkers specific for persistent S. aureus strains as well as to use the spectra to differentiate colonizing, persisting and aggressive S. aureus strains.
What techniques and methods are used?
- biophotonic techniques that involve interaction of light with biological samples, in particular Raman spectroscopy, wide-field and fluorescence microscopy; - basic microbiological methods, such as cultivation of bacteria, growth measurement, and fluorescence staining: - basic methods of spectroscopic and image analysis to evaluate the data
What is the role of the student?
What are the tasks expected to be accomplished by the student?
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary reading will be send prior to project start, in weekly seminars current research topics from the group and theoretical basics will be discussed. All experimental techniques and
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
First experience with lab work (pipetting, handling of buffers), basic knowledge in microscopy as well as basic knowledge in data analysis and statistical analysis is an advantage. The work will be carried out in a laboratory with gene safety level 2 where also patients' samples are analyzed. Therefore, a hepatitis B immunization status is required and needs to be provided as copy for working for insurance and safety reasons. Furthermore, due to the German safety regularities associated with working with pathogens, students should be at least 18 years of age and may not have a severe disease that suppresses the immune system function Detailed knowledge in biophotonic techniques is not required; however, a strong interest and motivation for working on this interdisciplinary project and to learn new theory and methods and read background literature independently is expected. Prior experienceof general lab work is beneficial.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Tuchscherr et al.; 2011; EMBO Mol Med 3; 129-141; doi: 10.1002/emmm.201100123
- Garzoni& Kelly; 2009; Trend in Microbiology 17 (2); doi: 10.1016/j.tim.2008.11.005
- Große et al.; 2015; Anal Chem 87; 2137-2142; doi: 10.1021/ac503316s
- 129-141 doi 10.1002/emmm.201100123
- Neugebauer et al.; 2007; ChemPhysChem 8; 124-137; doi: 10.1002/cphc.200600507
- Harz et al.; 2009; Cytometry 75A; 104-113; doi: 10.1002/cyto.a.20682
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