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Genetic and pharmacogenomic basis of chronic autoimmune and inflammatory diseases
Slovenia (SloMSIC) - University of Maribor, Maribor
University of Maribor, Faculty of Medicine, Centre for Human Molecular Genetics and Pharmacogenomics Taborska ulica 8, SI-2000 Maribor, Slovenia
Prof. Dr. Uros Potocnik
Katja Repnik, Stasa Jurgec, Helena Sabina Celesnik, Gregor Jezernik and Mario Gorenjak
English, Slovene, Serbian, Croatian,
Type of Research Project
- Basic science
What is the background of the project?
Genome wide association studies (GWAs) of autoimmune and inflammatory diseases identified many single nucleotide polymorphisms (SNPs) associated with disease susceptibility. However, the majority of disease associated SNPs are located within non-coding regions of genome. Linking disease associated non-coding SNPs to genes they affect, identification of causal variants and explaining molecular mechanism by which non-coding SNPs affect disease genes, is the major challenge for post GWAs genetic studies of complex diseases, such as inflammatory bowel disease (IBD) and asthma, in order to provide new molecular diagnostic and pharmacogenetic markers as well as molecular targets for novel drugs design for better prevention and treatment of complex diseases. What is the aim of the project? Our current research aims to link disease-associated SNPs with disease-causative genes and to discover the mechanisms by which non-coding SNPs affect genes and proteins involved in disease pathogenesis. Specifically, this project will focus on DNA genotyping, mRNA expression and protein identification of target genes in peripheral blood mononuclear cell (PBMC) from well categorized adult patients with IBD and asthma on different therapies with defined outcomes. Further, genetic data will be compared against response to treatment, to discover new pharmacogenomics biomarkers for efficient personalized treatment of IBD and asthma. What techniques and methods will be used? Isolation of DNA, RNA and proteins from PBMCs of patients with Inflammatory bowel diseases and asthma using TRI isolation reagent. (ii) Genotyping of selected SNPs with PCR-HRM, PCR-RFLP or TaqMan
What is the aim of the project?
genetic data will be compared against response to treatment, to discover new pharmacogenomics biomarkers for efficient personalized treatment of IBD and asthma.
What techniques and methods are used?
Isolation of DNA, RNA and proteins from PBMCs of patients with Inflammatory bowel diseases and asthma using TRI isolation reagent. (ii) Genotyping of selected SNPs with PCR-HRM, PCR-RFLP or TaqMan
What is the role of the student?
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
student will assist experienced researcher (preparing buffers - solutions and laboratory material - calculations), data entry and processing - DNA - RNA and protein isolation - PCR reaction. Afterwards student will be encouraged to work independently.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic practical skills of working in laboratory (pipetting, chemical calculation, etc). Basic statistical literacy. Advanced computer and internet literacy. Spoken English language should be at least of B2 level (CEFR scale). Basic knowledge of genetics, biochemistry and molecular biology. Self-iniative. Passion for scientific research work. Scientific report after the exchange is encouraged, but not demanded.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
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