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Analysis of serum matrix metalloproteinase activities in neurodegenerative disorders
Poland (IFMSA-Poland) - Medical University of Lublin, Lublin
Department of Medical Chemistry, Medical University of Lublin, Poland
Jacek Kurzepa, M.D., PhD
Anna Boguszewska-Czubara, PhD
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases responsible for degradation of extracellular proteins. Two of them, MMP-2 and MMP-9, are involved in the pathogenesis of numerous diseases within central nervous system (CNS). Their role in physiology and pathology of CNS is extensively studied recently. MMP-9 has been found to be involved in inflammation and therefore facilitating the blood-brain barrier destruction. It also enhances the influx of leukocytes and activates several pro-inflammatory cytokines and growth factors. The important role of MMPs has been underlined in the pathogenesis of meningitis, multiple sclerosis and neurodegenerative diseases. There are many publications, which show that metalloproteinases are involved in damaging the CNS, but quite recently their involvement in CNS development as well as in repair processes have been proved.
What is the aim of the project?
The aim of the project is to find out if elevation in MMPs activity (mainly MMP-2 and MMP-9) in blood serum is correlated with impairment in cognitive processes and may be negative predictor of clinical out-come for patients with neurodegenerative disorders (like Parkinson s or Alzheimer s disease).
What techniques and methods are used?
Gelatin zymography ? the technique based on SDS-PAGE (electrophoresis) with a gelatin incorporated into the gel as a substrate for MMP-2 and MMP-9. After electrophoresis enzymes digest gelatin results in appearance of bright bands on blue (after staining) background. ELISA ? enzyme-linked immunosorbent assay with usage of standard MMP-s kits.
What is the role of the student?
What are the tasks expected to be accomplished by the student?
Participation and self-development.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No special skills.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
- Singh D. Srivastava SK. Chaudhuri TK. Upadhyay G. Multifaceted role of matrix metalloproteinases (MMPs). Front Mol Biosci. 2015 May 13;2:19.
- Kaplan A. Spiller KJ. Towne C. Kanning KC. Choe GT. Geber A. Akay T. Aebischer P. Henderson CE. Neuronal matrix metalloproteinase-9 is a determinant of selective neurodegeneration. Neuron. 2014 Jan 22;81(2):333-48.
- Terni B. Ferrer I. Abnormal Expression and Distribution of MMP2 at Initial Stages of Alzheimer's Disease-Related Pathology. J Alzheimers Dis. 2015;46(2):461-9.
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