Projects
Name
The use of recombinant proteins in the study of neurological diseases. Epsilon toxin from Clostridium perfringens and its ability to bind myelin
University
Catalonia (AECS) - University of Barcelona (UB)- Hospital de Bellvitge, Barcelona
Domain
Pathology
Departement
Department of Pathology and Experimental Therapeutics
Head
Dr. Teresa Vinuesa
Tutor
Dr. Juan Blasi and Carles Solsona
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No Yes No No No Yes No No No No No No
Type of Research Project
- Basic science
What is the background of the project?
Clostridial toxins have been a useful tool to understand cellular and molecular mechanism, especially in the membrane traffic and secretion. Recently, a possible role in the onset of multiple sclerosis (MS) has been proposed, in particular for epsilon toxin, as a possible causative environmental factor.
The main aim is to study and understand the specific cytotoxic mechanism of epsilon toxin, its ability to cross de blood brain barrier and bind myelin and its possible relationship with the onset of MS.
The techniques and methods involve Cellular and Molecular Biology, Biochemistry and Electrophysiological approaches, and may include recombinant protein production, immunofluorescence, cell culture, protein characterization and two-electrode voltage patch clamp among others, but the exact techniques and methods to be used will depend on the ongoing of the main project of the lab.
What is the aim of the project?
The main aim is to study and understand the specific cytotoxic mechanism of epsilon toxin, its ability to cross de blood brain barrier and bind myelin and its possible relationship with the onset of MS.
What techniques and methods are used?
The techniques and methods involve Cellular and Molecular Biology, Biochemistry and Electrophysiological approaches, and may include recombinant protein production, immunofluorescence, cell culture, protein characterization and two-electrode voltage patch clamp among others, but the exact techniques and methods to be used will depend on the ongoing of the main project of the lab.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student would be involved in the daily activity of a Cell Biology and Physiology laboratory. After an initial description and discussion of the main lines and goals of the project, the student will participate in the experimental approach using the techniques and the methodology used at these moments in the lab.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Scientific bibliography (papers, book chapters) and seminars will be provided
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
- The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student should have a basic knowledge in Cellullar and Molecular Biology, Biochemistry and Physiology. We consider a m
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Pre-Medical students from the American-British system
- Students in biomedical fields
Articles
- http://www.ncbi.nlm.nih.gov/pubmed/25096579
- http://www.ncbi.nlm.nih.gov/pubmed/24123415
- http://www.ncbi.nlm.nih.gov/pubmed/26452234
- http://www.ncbi.nlm.nih.gov/pubmed/25013927
- http://www.ncbi.nlm.nih.gov/pubmed/22264388