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(Iasi) IMMUNOPHENOTYPING BY FLOW CYTOMETRY USED FOR THE DIAGNOSIS OF HEMATOLOGICAL MALIGNANCIES
Romania (FASMR) - University of Medicine and Pharmacy "Gr.T. Popa", Iasi
Oncology Department, Regional Institute of Oncology, Iasi, Laboratory of Molecular Diagnosis, Flow cytometry Department
Assistant teacher Florin Zugun Eloae MD, PhD
Assistant teacher Florin Zugun Eloae MD, PhD; Biolog. Mihaela Zlei, PhD
Type of Research Project
- Basic science
What is the background of the project?
In spite of the comprehensive knowledge that we have recently gained on the human genome, we are far from having a unified opinion about how biostructures (responsible for the cell function and metabolic pathways compartmentalization) are generated. In this context, flow cytometry offers the possibility of a quick evaluation of multiple cell parameters in heterogeneous cell suspensions. Based on comparative analysis of data provided by the assessment of complex cell suspensions collected both from healthy individuals and from patients with different pathologies, we can build phenotypic profiles that reflect the structural and functional changes occuring in different diseases.
What is the aim of the project?
The aim of the project is to correlate data generated by techniques such as multiparametric flow cytometry, molecular biology, cytogenetics, medical imaging, and microscopy in order to diagnose a broad range of hematological malignancies (acute leukemias, chronic lympho-proliferative diseases), predict their evolution, help the clinician in choosing the optimal treatment for each patient, as well as identify possible therapeutic targets in the pharmacological research.
What techniques and methods are used?
Immunophenotyping techniques (sample staining with fluorochrome conjugated monoclonal antibodies, erythrocyte lysing, sample washings, sample acquisition, data analysis, gating strategy, cell population assingment, cell differentiation assessment), software tools for multiparametric flow cytometry data interpretation, (FACSDiva, BD Becton Dickinson, Kaluza, Beckman Coulter, Infinicyt, Cytognos)
What is the role of the student?
What are the tasks expected to be accomplished by the student?
- Demonstration of flow cytometry principle (practical sesions on cytometers with three lasers, twelve parameters: FacsAriaIII, BD Biosciences, Navios, Beckman Coulter, and Gallios, Beckman Coulter) - Theoretical, general presentation of hematopoietic cell lineage immunophenotype and maturation profiles - Explanations of fluorochrome-conjugated antibodies; - Practical sessions: immunophenotyping of a B cell chronic lymphoid leukemia sample. - Practical sessions: analysis of a multiple myeloma bone marrow sample. - Practical sessions: Immunophenotyping an acute leukemia sample.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge about immunology and hematology.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
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