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Synthesis and biological evaluation of chloramphenicol conjugates with polyamines: Promising agents with antibacterial and anticancer activity
Greece (HelMSIC) - University of Patras, Patra
Department of Biochemistry, School of Medicine, University of Patras
Prof. Dionysios Drainas
Prof. Dimitrios Kalpaxis, MSc Georgia Kournoutou
English (required) , Italian / German (accepted)
Type of Research Project
- Basic science
What is the background of the project?
Background information: Chloramphenicol is a broad spectrum antibiotic, which inhibits protein synthesis by binding to the catalytic center of the ribosome and blocking essential ribosomal functions. Nevertheless, the clinical use of chloramphenicol is limited due to its adverse effects that include bone marrow depression and aplastic anemia. Therefore, chloramphenicol has become a subject of synthetic approaches aiming to optimize its pharmaceutical profile. What is the aim of the project? Aim of the project is the biological evaluation of a series of chloramphenicol-polyamine conjugates. We envisage polyamine moiety offering additional binding sites to the constructs as well as increase susceptibility in Escherichia coli and other Gram-negative or ?positive bacteria, given that polycations are able to perturb the outer membrane of bacteria. What techniques and methods will be used? a. Biochemical preparations of 70S ribosomes from Escherichia coli K12 cells and calculation of the affinity of the synthesized agents for the ribosome, using kinetic analysis b. Measurement of the Staphylococcus aureus and Escherichia coli cells sensitivity to the synthesized agents, by estimating the IC50 value for each strain. c. Toxicity assays in human peripheral blood cells and leukemic cell lines, using flow cytometry d. Docking of the synthesized agents into the 50S ribosomal subunit satructure and Molecular Dynamic Simulations
What is the aim of the project?
What techniques and methods are used?
What is the role of the student?
- If the project is clinical
- the student will take active part in the clinical examination
What are the tasks expected to be accomplished by the student?
Assist in the Lab Work of the project
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Pre-Medical students from the American-British system
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