Projects
Name
Recombinant vaccine against tuberculosis development and testing.
University
Federal University of Goiania (UFG)
Domain
Immunology
Departement
epartment of Microbiology, Immunology,Parasitology and Pathology
Head
Carla Afonso Bitencourt Braga
Tutor
Andre Kipnis
Languages
Portuguese, Spanish, English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No yes yes yes yes yes yes yes yes yes No
Type of Research Project
- Basic science
What is the background of the project?
Tuberculosis is a highly endemic disease in 22 countries of the world. It is caused byMycobacterium tuberculosis, and is the second most frequent cause of death by an infectious disease, killing about 1.3 million people each year. Despite having a reliable diagnose and treatment, its rate has remained high in this century for different reasons, one being the HIV coinfection. A safe and widely used vaccine for tuberculosis is being using since the 1920s, the attenuated M. bovis BCG. This vaccine is efficient to prevent severe tuberculosis in children but looses its protection when individuals reach their teenage. Consequently there is a need to develop improved vaccines for BCG.
What is the aim of the project?
Our aims are to develop recombinant BCG vaccines, by inserting additional andimportant M. tuberculosis genes in the M. bovis BCG, analyze the immunological responses of this recombinant vaccine in mice.
What techniques and methods are used?
The techniques and methods that are going to be used are the molecular recombination and cloning, performing primer design, PCR amplifications, gene cloning and transformation in Escherichia coli and screening the right plasmid constructs, immunological assays, such as ELISAs, mouse vaccination, cell preparation and analysis by flow cytometry.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The techniques and methods that are going to be used are the molecular recombination and cloning, performing primer design, PCR amplifications, gene cloning and transformation in Escherichia coli and screening the right plasmid constructs, immunological assays, such as ELISAs, mouse vaccination, cell preparation and analysis by flow cytometry.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
0
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Some understanding of microbiology and organic chemistry are desired. Molecularbiology and infectious disease pathogenesis would also be very useful. We expect a student that have lots of interest for any kind of science, have initiative and proactivity as well as one than has understanding and wiling to accept differences that may appear between individuals in the lab.
Are there any legal limitations in the student’s involvement
No
Hours
8
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
Articles