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Reverse genetics in Drosophila: RNAi screen for molecular factors important for stem cell self-renewal, division and differentiation
Germany (bvmd) - Universitaet zu Koeln, Cologne
Molekulare Zellbiologie, Institut I for Anatomy,Kerpener Str. 62, Geb.37, 50937 Koeln Germany
Prof. Dr. Andreas Wodarz, firstname.lastname@example.org
Dr. Manu Tiwari
Type of Research Project
- Basic science
What is the background of the project?
Stem cells (SCs) are self-renewing cells whose division ultimately results in terminally differentiated progeny. Their self-renewal property with each division is the key to tissue formation and is of immense importance for tissue regeneration followinginjury. This short exchange project envisages the use of Drosophila melanogaster ovarian germline SCs to study novel self-renewal genes. It entails the use of Drosophila flies with RNAinterference (RNAi: gene knock-down) directed against specific genes; when combined with cell-specific expression (Drosophila-Gal4 system), these flies express RNAi in the selected cells. Next, by using immunohistochemistry and microsocopy, the expression of specific antigens signifying SCs and their progeny is detected.
What is the aim of the project?
Aim of the project: To find novel genes involved in germline SC self-renewal and differentiation: If a gene X is involved in SC self-renewal, division or differentiation, then silencing the activity of that gene will cause fluctuation in SC activity; this should reflect in the number(s) and type(s) of the SC progeny, which can be detected through immunohistochemistry.
What techniques and methods are used?
What techniques and methods will be used? Drosophila genetics, RNAi, Immunohistochemistry,Light and Confocal microscopy. 1. Generation of Drosophila flies lacking the activity of specific gene(s) using Drosophila genetics and RNAi. 2. Assessment of the division/differentiation/behaviour of Germline Stem cells in these flies by immunofluorescence staining followed by light and confocal microscopy.
What is the role of the student?
What are the tasks expected to be accomplished by the student?
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No such pre-requisites. A brief, written summary of findings during the project is required (format of the summary will be provided during the project) For the use of students considering participating in the project, further information can be found from the following articles: Shahab J et al., Biol Open. 2015, 4: 528-541. Elliott DA and Brand AH. Methods Mol Biol. 2008, 420: 79-95. Novina CD and Sharp PA.Nature.2004, 430: 161-164.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
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