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Studies on the presence of nanovesicles in Sertoli cells and on the microencapsulated Sertoli cells
Universita degli studi di Perugia
Department of Experimental Medicine, Section of Bioscience and Embryology
Dott. Giovanni Luca Dott. Mario Calvitti, Dott.ssa Francesca Mancuso, Dott. Maria Bodo
Type of Research Project
- Basic science
What is the background of the project?
In many recent studies, it has been demonstrated that intercellular communications are related to small vesicles. These extracellular nanovesicles (<120 nm in size), such as exosomes, can be found in biological fluids such as blood, urine, and ascites influencing physiological and pathological processes, including immune response. A variety of normal cells or tumor cells in culture have been shown to secrete these nanovesicles into the cell culture medium. Until now, it has not yet been demonstrated the presence of these nanovesicles in Sertoli cells (SC). Nanovesicles have drawn wide attentions in both diagnostic and therapeutic applications, since they are considered to shuttle biological signals intercellularly .
What is the aim of the project?
1) to study the production of nanovesicles either in SC monolayer (nv-SC), focusing the attention on the characterization of the different immunoregulatory factors possibly delivered by nv-SC; 2) to study the potential immunomodulatory/immunoregulatory effects of nv-SC as therapeutic tool in autoimmune disease such as type 1 diabetes in animal model.
What techniques and methods are used?
For this study will be used the following techniques: - cell culture - Immunostaining - RNA estraction - Real Time PCR - Western Blotting - ELISA Kit
What is the role of the student?
- The student will mainly observe
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will provide to: Sertoli cell isolation Nanovesicles isolation and characterization Micro-RNA detection and analysis The student will acquire knowledge on Sertoli cell culture and on techniques of molecular biology
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
It is necessary a knowledge of basic sciences. For the use of students considering participating in the project, further information can be found from the following articles: - G. Luca, et al. Long-Term Stability, Functional Competence And Safety Of Microencapsulated Specific Pathogen-Free (Spf) Neonatal Porcine Sertoli Cells: A Potential Product For Cell Transplant Therapy. Xenotransplantation. 2015 Jul; 22(4):273-83 - Romani R et al. Stem cells from human amniotic fluid exert immunoregulatory function via secreted indoleamine 2,3-dioxygenase1. J Cell Mol Med. 2015 Jul;19(7):1593-605 - Takayuki Kogure and Tushar Patel. Isolation of Extracellular Nanovesicle MicroRNA from Liver Cancer Cells in Culture. Circulating MicroRNAs: Methods and Protocols, Methods in Molecular Biology, vol. 1024
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Pre-Medical students from the American-British system - Students in biomedical fields
- G. Luca; et al. Long-Term Stability; Functional Competence And Safety Of Microencapsulated Specific Pathogen-Free (Spf) Neonatal Porcine Sertoli Cells: A Potential Product For Cell Transplant Therapy. Xenotransplantation. 2015 Jul; 22(4):273-83
- Romani R et al. Stem cells from human amniotic fluid exert immunoregulatory function via secreted indoleamine 2;3-dioxygenase1. J Cell Mol Med. 2015 Jul;19(7):1593-605
- Takayuki Kogure and Tushar Patel. Isolation of Extracellular Nanovesicle MicroRNA from Liver Cancer Cells in Culture. Circulating MicroRNAs: Methods and Protocols; Methods in Molecular Biology; vol. 1024
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