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Investigation of embryonic stem cell marker expression pattern in Inflammatory Bowel Disease
Greece (HelMSIC) - National and Kapodistrian University of Athens, Athens
Department of Basic Medical Sciences, Laboratory of Biology,University of Athens
Prof. E. Marinos
Assist Prof M. Gazouli
Type of Research Project
- Basic science
What is the background of the project?
Developmentally early cells, including hematopoietic stem progenitor cells (HSPCs),mesenchymal stem cells (MSCs), endothelial progenitor cells (EPCs), and very small embryonic-like stem cells (VSELs), are mobilized into peripheral blood (PB) in response to tissue/organ injury. The aim of the project is to determine whether these cells are mobilized into PB in patients with Crohn's disease (CD) and its correlation with the injured tissues. Methods that will be used include DNA/RNA isolation, protein purification, PCR, Real time PCR, reverse transcription, immunohistochemistry, western blots.
What is the aim of the project?
What techniques and methods are used?
What is the role of the student?
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Assist in the Lab Work of the project
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
For the use of students considering participating in the project, further information can be found from the following articles (please add specific articles): Stem cells as potential targeted therapy for inflammatory bowel disease. Gazouli M, Roubelakis MG, Theodoropoulos GE. Inflamm Bowel Dis. 2014 May;20(5):952-5 OCT4 spliced variant OCT4B1 is expressed in human colorectal cancer. Gazouli M, Roubelakis MG, Theodoropoulos GE, Papailiou J, Vaiopoulou A, Pappa KI, Nikiteas N, Anagnou NP. Mol Carcinog. 2012 Feb;51(2):165-73. c/o WMA B.P, 63,01212 Ferney-Voltaire CDEX ?FRANCE Tel. +33 (450) 04 47 59 Fax. +33 (450) 40 59 37 www.ifmsa.org Inflammatory bowel disease: recent advances on genetics and innate immunity. Karantanos T, Gazouli M. Ann Gastroenterol. 2011;24(3):164-172.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
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