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Personalization of isoniazide treatment of tuberculosis patients by pharmacogenetic approach
Portugal (PorMSIC) - University of Coimbra, Coimbra
Department of Medical Genetics, Faculty of Medicine, University of Coimbra
Fernando Jeus Regateiro
Henriqueta Coimbra Silva and Celeste Alcobia
English or Portuguese
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
In the 21st century, tuberculosis is still a leading cause of death from an infectious disease worldwide. Isoniazid (INH) is a pivotal agent in the treatment of tuberculosis, included in all therapeutic protocols, but it is also the most prevalent cause of drug-induced hepatotoxicity (DILI), an adverse effect occurring in 10-36% of patients and that may be fatal. Genotyping of polymorphisms of NAT2 gene, encoding N-acetyltransferase 2, the major INH-metabolizing enzyme, allows identifying individuals with different pharmacokinetic profiles. Slow acetylators, accounting for 50% of patients in Caucasian populations, have increased susceptibility to DILI.
What is the aim of the project?
The major aim of the project is to evaluate the repercussion of personalization of INH dosing by genotyping in the management of tuberculosis patients.
What techniques and methods are used?
Lab work genotyping patients in the Laboratory of the Medical Genetic Department. Genotyping will include DNA extraction, PCR, Sanger sequencing and data interpretation.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Students will participate in patients consultations with the clinical tutor, Drª Celeste Alcobia, a specialist in Pneumology, and will perform lab work in the Genetic Department. The student has to participate in consultations of patients with pulmonary tuberculosis, where he will be assisted by Drª Celeste, once a week. The student is also expected to perform lab work genotyping patients, in the Laboratory of the Medical Genetic Department. Genotyping will include DNA extraction, PCR, sequencing and data interpretation. At the end the student is expected to perform an oral presentation about the work.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
It will be more interesting for the student if he has already done some clinical courses including Pneumology. The student must like lab work
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
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